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Aseptic cultivating method for newly-hatched silkworm

A breeding method and technology of ant silkworms, which are applied in animal husbandry and other fields, can solve problems such as moldy feed and silkworm body weakness, and achieve the effect of convenient operation and simple method

Inactive Publication Date: 2005-01-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Silkworm artificial feed is rich in nutrients, high in water content, and high temperature and humidity requirements in the breeding environment, which can easily cause mold and deterioration of the feed, and cause silkworm body weakness and silkworm disease.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] The ordinary silkworm eggs were accelerated to the greening stage, and divided into two groups one day before the hatching of the ant silkworms, and the two groups of silkworm egg surfaces were disinfected respectively. Among them, the first group was soaked in 4% formaldehyde for 15 minutes, and the silkworm eggs were removed. Then soak in 95% ethanol for 1 minute. The second group was soaked with 4% formaldehyde for 10 minutes, and then soaked with 95% ethanol for 4 minutes. After the two groups of silkworm eggs that have been sterilized are dried naturally in a sterile place, they are supplemented and accelerated according to the conventional method until the silkworm eggs hatch, and sterile ant silkworms are obtained, and the ants are collected for rearing.

[0009] The hair-thinning rate and growth and development of the above-mentioned first and second groups of sterile ant silkworms after disinfection treatment were compared with those of the control group withou...

Embodiment 2

[0014] The first and second groups described in Example 1 were compared with the hatching rate of the control group, and the results are shown in Table 2. As can be seen from Table 2, the egg surface disinfection treatment has almost no impact on the hatching rate.

[0015] Table 2 Hatch rate of different egg surface disinfection

[0016] one day hatch rate

Embodiment 3

[0018] Two groups of aseptic ant silkworms hatched through egg surface disinfection are collected in the ants and raised in embodiment 1, and the mildew situation of its artificial feed is investigated. Silkworm test area) comparison, the results are shown in Table 3. It was found that the mildew of the sterile ant silkworm was 42 hours later than that of the control group, which indicated that unsterilized eggs, egg shells and ant silkworm had a direct impact on the mildew of the artificial feed.

[0019] Table 3 The relationship between different egg disinfection and artificial feed mildew

[0020]

[0021] It can be clearly seen from Table 3 that the mildew time of the artificial feed can be delayed after the egg surface is sterilized for the transferred green eggs. It takes more than 6 days from harvesting ants to discovering mildew spots, and the silkworms have developed to the middle and late stages of the second instar. Therefore, it is co...

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Abstract

An aseptic culture method for silkworm larvae includes disinfecting silkworm ova one day before they ar incubated by immersing in formaldehyde solution for 10-15 min and then in alcohol for 1-4 min, drying in air, inculating to obtain larvae and culturing.

Description

technical field [0001] The invention relates to a method for cultivating sterile ant silkworms of silkworms. Background technique [0002] With the development and research of silkworms as bioreactors to produce a variety of biological products, the demand for silkworms is increasing. Traditional sericulture is not only limited by seasons and regions, but also competes for land with grain and other economic crops. Labor conflicts are also increasing. Artificial feed can provide silkworms anytime and anywhere, alleviating the contradiction between planting mulberry and growing grain and other economic crops for land and labor, greatly improving labor productivity and reducing labor intensity. Silkworm artificial feed is rich in nutrients, high in water content, and high temperature and humidity requirements in the breeding environment, which can easily cause mold and deterioration of the feed, and cause silkworm body weakness and silkworm disease. ...

Claims

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Application Information

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IPC IPC(8): A01K67/04
Inventor 缪云根
Owner ZHEJIANG UNIV
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