Pouring type biologic reactor system in vitro constructure of dual active skin

Abstract

The invention is a double-layer active skin-composed in vitro pouring bioreactor system, including a liquid-adding device, a pouring bioreactor, a liquid-collecting device, a liquid reflux and a gas supply device; the liquid-adding device is connected with a liquid inlet of the pouring bioreactor, the liquid-collecting device is connected with a liquid outlet of the pouring bioreactor to collect the culture medium after culture, the liquid reflux is connected with the liquid -adding and -collecting devices through pipelines, and the gas supply device is connected with a gas inlet of the pouring bioreactor through pipeline; the bracket or cell material compound for composing the skin is smoothed out on a multiporous supporting plate of the pouring bioreactor. It has simple structure, easy to enlarge and can make wet-hot sterilization, maintain axenic environment for a long time and primely simulate in vivo growth environment, implementing the course from vaccinating to derma composing to the entire double-layer active skin composing in vitro by pidermis.

Description

technical field [0001] The invention relates to a bioreactor, in particular to a perfusion bioreactor for constructing double-layer active skin in vitro in medicine and tissue engineering. Background technique [0002] Skin is the largest tissue in the human body, with a surface area of ​​about 1.5-2.0m 2 . As the outermost barrier of the human body, the skin is also the most vulnerable tissue. Approximately 60,000-80,000 burn patients require skin grafts each year. [0003] Currently used autologous skin grafts, allogeneic skin grafts, and xenogeneic skin grafts have problems such as insufficient donor sites, immune rejection, and disease transmission. The emergence of tissue-engineered skin has injected new vitality into skin transplantation. [0004] The research on tissue-engineered skin began in the 1970s. So far, various skin substitutes with clinical value have been developed, which can be divided into epidermal substitutes, dermal substitutes and composite skin s...

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Problems solved by technology

[0009] The technical problem to be solved in the present invention is to disclose a perfusion bioreactor system constructed in vitro with double-layer active skin, so as to overcome the shortcomings of the prior art that the structure is complex, the in vivo growth environment cannot be simulated, and it is not easy to scale up, so as to meet the requirements of clinical application. need

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