Preparation process of diglyceride

Abstract

The invention discloses a method of preparing diglyceride, including the following steps: (1) in a raw material vat, adding acyl receptor containing glyceryl, fatty acid donor and hydrophilic carrier silica gel, and stirring; (2) under the catalytic action of immobilized lipase, the acyl receptor reacts with the fatty acid to generate diglyceride and side-products; (3) after vacuum dehydration or lower alcohol elimination, returning the reaction products to the raw material vat to repeat operation in steps (1)-(3); (4) separating. It solves a series of problems caused by the coating of enzyme by glycerol, improving the catalytic effect of fatty acid.

Description

technical field [0001] The invention relates to a preparation method of diglyceride. Background technique [0002] Diacylglycerol is a glyceride molecule with only two fatty acid chains. Diacylglycerol can be used as an emulsifier, a fat plasticity modifier or as a base for food, medicine, cosmetics, etc. Studies in recent years have found that the absorption and metabolism patterns of diglyceride and triglyceride are different. Diglyceride is more easily converted into energy and utilized in the body, while triglyceride is easier to accumulate in the body. Consuming fats containing diglycerides has the effect of reducing weight, so diglycerides can be eaten as a healthy fat. [0003] Esterification and transesterification are important methods for the production of diglycerides, which use glycerol or monoglycerides as fatty acid acceptors, fatty acids or fatty acid derivatives as fatty acid donors, and react through chemical catalysis or enzyme catalysis. The product cont...

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Problems solved by technology

[0004] Glycerol is the most commonly used acyl acceptor for the synthesis of diglycerides. Glycerol is a polar molecule. Under normal temperature and normal pressure enzyme reaction conditions, the compatibility between glycerol and fatty acid donor substances is very poor. Mix glycerol and fatty acid donors When in contact with lipase afterward, the polar glycerol molecules tend to be wrapped on the lipase, resulting in a serious reduction in the catalytic efficiency of the enzyme; glycerol has strong water absorption, and the glycerol adsorbed by the immobilized lipase can retain a certain amount of water. A higher water concentration is formed around the lipase, which seriously affects the speed of the esterification reaction

[0005] U.S. Patent No. 6,361,980B2 discloses a method for utilizing fatty acid donors and glycerol esterification to generate diglycerides. The method utilizes free glycerol and fatty acid donors to react, and requires that the residence time of the reaction substrate pass through the immobilized lipase reactor should not be longer. More than 120 seconds, part of the reason is that the shear force at high flow rates reduces the encapsulation of glycerol on the enzyme. Therefore, this method has high requirements for the enzyme reactor and the transfer pump, and the production is difficult to realize, especially when the enzyme reactor When the size of the immobilized enzyme is large; at the same time, the high shear force has a negative side to the immobilized enzyme

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