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Fast and semiquantitative detection method for Clenbuterol

A semi-quantitative detection and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult on-site adoption

Inactive Publication Date: 2004-11-17
SOUTH CHINA AGRI UNIV
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  • Abstract
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Problems solved by technology

However, this method still requires some equipment such as a microplate reader, and the analysis time is 1-3 hours, so it is difficult to adopt in the field

Method used

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  • Fast and semiquantitative detection method for Clenbuterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: 1 detection line + 2 reference lines test paper to detect clenbuterol in pig urine

[0047] 1. Synthesis of clenbuterol-coupled antigen

[0048] Clenbuterol conjugated antigens are divided into antigens for immunization and antigens for detection. The former is used to immunize animals, and the latter is used for antibody detection and screening in antibody preparation and colloidal gold labeling or detection line coating in test paper preparation.

[0049] Antigen preparation for immunization: Accurately weigh 22 mg of clenbuterol and dissolve in 2 mL of hydrochloric acid solution with pH 1.5-2.0. Add 2 mL of sodium nitrite solution with pH 2.0 and react for 20 min. At the same time, weigh 100mg bovine serum albumin and dissolve it in 5mL pH7.4, 0.1mol / L phosphate buffer solution, add the reacted clenbuterol diazonium salt, and adjust the pH value to 7.5, after 12 hours of reaction at 4°C. Dialyze the reaction solution with pH 7.4, 0.01 mol / L PBS solution...

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Abstract

The invention refers to a kind of Clenbuterol Hydrochloride semiquantitative quick measuring method. The invention pastes solution sample absorbing part, colloidal gold label part, measurement reaction part and water absorbing part on the back lining of the reagent paper. The measurement part is enclosed with 1-3 stripes of Clenbuterol Hydrochloride antibodies or 1-3 stripes of measuring antigens which are sued as measuring lines, at the same time, it is enclosed with 1-3 stripes of the second species animal protein resisting IgG which are used as reference lines. When combining, the measuring lines and the reference lines couldn't more than 1 stripe at the same time, the compound lines are 2-4 stripes. The quick measuring reagent paper has a strong specificity, the sensitivity can reach 0.09ng.mL, it can be used under temperature of 4-40 degrees, the result can be observed after two minutes.

Description

technical field [0001] The invention relates to a method for detecting clenbuterol (CLB). Background technique [0002] Clenbuterol, also known as clenbuterol hydrochloride, or clenbuterol, commonly known as "clenbuterol", was originally used to treat diseases such as asthma. After it was found that it could significantly increase animal production and lean meat rate, it was once used as a Feed additives are widely used in animal husbandry. However, it is highly toxic and easily remains in animal tissues, and people often cause poisoning due to ingesting its animal food. Although my country banned its use in feed in 1997 after Europe and the United States, the phenomenon of illegal abuse still exists due to economic interests. According to the information released by the Ministry of Agriculture on the Internet, some unscrupulous traders make clenbuterol into small packages for retail or sell it in combination with feed. Therefore, when grasping sou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
Inventor 孙远明雷红涛潘科黄丽黄晓钰谌国莲吴青
Owner SOUTH CHINA AGRI UNIV
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