Immune colloidal gold reagent for detecting stilboestrol and preparation method thereof

Abstract

The invention is a kind of immune colloidal gold reagent for measuring the ethene estraphenol and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of ethene estraphenol resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by ethene estraphenol-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for detecting diethylstilbestrol mainly include enzyme-linked immunosorbent assay (EIA), radioimmunoassay (RIA), immunoaffinity chromatography (IAC), and gas / mass spectrometry. The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection Higher, single-person detection cannot be realized. The disadvantages of radioimmunoassay are: professional equipment is needed, and the equipment is expensive; operators need professional training; the operation process is complicated and demanding; reagents ...

AI Technical Summary

Problems solved by technology

The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection High, unable to achieve single-person detection

The disadvantages of radioimmunoassay are: professional equipment is needed, and the equipment is expensive; operators need professional training; the operation process is complicated and demanding; reagents need to be stored at low temperature; because the test involves radioactive Rarely used; high testing cost; long testing time

The disadvantages of immunoaffinity chromatography (IAC) are: it is necessary to prepare a special immunoaffinity column; operators need to undergo professional training; the operation process is complicated and demanding; the detection cost is high; the detection takes a long time; it is mainly used for sample processing , the application and research in residue analysis is still in the exploratory stage

The disadvantages of gas / mass spectrometry analysis method are: professional equipment is required, and the equipment is expensive; the sample pretreatment procedure is cumbersome and demanding; there are strict requirements for water and various solvents used in operation; the operation process is complicated , takes a long time; professionally trained technicians are required to operate, and operators must have rich relevant experience; operators must understand various interference factors that affect chromatographic analysis, and understand the advantages and disadvantages of the pretreatment methods used in order to obtain reliable results. Analytical results; expensive instruments and equipment are needed, and it is difficult to popularize in cities and small and medium-sized enterprises; the requirements for instrument maintenance are high, and the quality of maintenance directly affects the accuracy of the analysis results; the detection cost is high