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Light and heavy chain variable region genes of monoclonal antibody CAb-2 against human colorectal carcinoma and their application

A monoclonal antibody, variable region technology, applied in applications, antibodies, genetic engineering, etc., can solve the problem of difficulty in obtaining high-affinity and high-specificity colorectal cancer monoclonal antibodies

Active Publication Date: 2004-11-17
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Due to the mutation or partial loss of tumor antigens, it is difficult to obtain high-affinity and high-specificity colorectal cancer monoclonal antibodies by this method

Method used

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  • Light and heavy chain variable region genes of monoclonal antibody CAb-2 against human colorectal carcinoma and their application
  • Light and heavy chain variable region genes of monoclonal antibody CAb-2 against human colorectal carcinoma and their application
  • Light and heavy chain variable region genes of monoclonal antibody CAb-2 against human colorectal carcinoma and their application

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Experimental program
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Embodiment Construction

[0015] (1) Cloning of light and heavy chain variable region genes of anti-human colorectal cancer monoclonal antibody CAb-2

[0016] The cell line used is a high-affinity, high-specificity mouse-derived anti-human colorectal cancer monoclonal antibody CAb-2 hybridization obtained by the inventors by using fresh colorectal cancer tissue homogenate to immunize mice and using traditional fusion methods tumor cell lines. The secreted antibody does not bind to tumor-associated antigens such as CEA, 17-1A, TAG-72, CEA, A33, etc., and is aimed at a new tumor-associated glycoprotein antigen with a molecular weight of about 200KD. Its secreted antibody molecular subtype is IgG1κ.

[0017] CAb-2 hybridoma cells (5×10 6 ), using guanidine isothiocyanate one-step method to extract total RNA, and take a small amount for quantification by UV spectrophotometer and detection by 1% formaldehyde-denaturing agarose gel electrophoresis. Subsequently, Oligo(dT)15 (Promega Company) was used as a...

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Abstract

The invention relates to monoclonal antibody CAb--2 heavy and light chain variable region gene of human carcinoma of large intestine, the encoded polypeptides by the said genes, and the use of the genes and the polypeptides in preparing the medicament for diagnosis or treatment of colon tumor or rectum tumor. By introducing a set of designed expanded primers, a strain of monoclonal antibody Cab-2 heavy and light chain variable region gene of human carcinoma of large intestine is cloned from the hybrid tumor cells. The gene can be applied in preparing the medicament for diagnosis or treatment of colon tumor or rectum tumor.

Description

technical field [0001] The present invention relates to a light and heavy chain variable region gene of an anti-human colorectal cancer monoclonal antibody CAb-2 and its encoded polypeptide, as well as the application of the gene and polypeptide in the preparation of medicaments for diagnosing and treating colorectal cancer . Background technique [0002] Colorectal cancer is a common malignant tumor that endangers the population. Its incidence rate ranks second in Europe and the United States, and ranks third in my country. With the continuous adjustment of people's diet structure, its incidence rate still has an upward trend. Therefore, it is of great significance to study the diagnosis and treatment of colorectal cancer. Although the current nuclear magnetic resonance and endoscopic technology continue to develop, the detection rate of early colorectal cancer tumors has increased, but there are still false positives and false negatives in clinical actual detection, that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P35/00C07K16/18C12N15/12G01N33/577
Inventor 陈志南邢金良杨向民张思河
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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