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Lactobacillus fixed cell in-situ separating-fermenting lactic-acid production process

A technology of immobilized cells and in-situ separation, which is applied in the field of lactic acid preparation, can solve the problems that the fermentation broth cannot pass through continuously and uniformly, block the entrance of the resin column, and reduce the number of active cells, and achieve long service life, low consumption, and fast proliferation Effect

Inactive Publication Date: 2004-09-15
TIANJIN GUARD T & D
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods still have the following disadvantages: during the fermentation process, the active cells in the fermentation broth are reduced, the conversion rate is low, the loss of lactic acid is too large, the energy consumption is large, and the cost is high.
The disadvantage of the former method is that the anion exchange resin absorbs lactic acid from the fermentation broth flowing through, and at the same time, bacteria and nutrients also flow into the resin column and are absorbed or retained. It will block the inlet of the resin column, so that the fermentation broth cannot pass through continuously and evenly.
The disadvantage of the latter method is that using methanol as the eluent for lactic acid elution is neither economical nor environmentally friendly.

Method used

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  • Lactobacillus fixed cell in-situ separating-fermenting lactic-acid production process
  • Lactobacillus fixed cell in-situ separating-fermenting lactic-acid production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A. Cell immobilization

[0026] (a) Preparation of bacterial suspension: Cultivate lactic acid bacteria to the end of exponential growth, centrifuge for 10 minutes with a centrifuge, the centrifugal force is 9000g, discard the supernatant, break up into bacterial suspension with sterilized normal saline of equal weight, Take 22-38.7 grams of the bacterial suspension;

[0027] (b) Preparation of the mixed solution of embedding agent and bacterial suspension: take 8-12 grams of polyvinyl alcohol and 2-4 grams of sodium alginate and dissolve them in 100 milliliters of distilled water, heat them in a water bath to dissolve them, mix them evenly and sterilize them, After being cooled to 40 DEG C, join in the bacterium suspension of (a) together with the 2% carrageenan distilled aqueous solution of 22~38.7 gram sterilizations, be bacterium suspension by weight ratio: 2% carrageenan solution: 8~12 Mixture of % polyvinyl alcohol and 2-4% sodium alginate = 1:1:3-5 mixed evenly;...

Embodiment 2

[0039] Select polyvinyl alcohol, sodium alginate, and carrageenan to prepare four different proportions of embedding agents according to the percentage concentrations in Table 1, which are used to make bead-shaped immobilized cell pellets. The following methods are used to test the embedded bacteria Bead strength: place embedded particles of equal particle size between two glass slides, put a weight on the glass slide until the particles are crushed, use the weight of the weight to characterize the strength of the particles, and measure its indicators. The results are shown in Table 1.

[0040] Table 1 Quality test of embedding agents prepared with different component concentrations

[0041] Sequence Polyethylene Seaweed Carrageenan Bacterial content Transformation rate Mechanical Particle phenomenon

[0042] No. Enol (%) Sodium Acid (%) (%) (%) Strength (g)

[0043] (%)

[0044] 1 6 1 2 0.1 90.20 <50 adhesion

[0045] 2 8 2 2 0.1 90.63 >50 ...

Embodiment 3

[0050] Lactic acid was produced by the technique of Example 1, and the immobilized lactic acid bacterial beads of the present invention were repeatedly used three times. Under the exact same conditions, unimmobilized lactic acid bacteria beads were used for production. The product concentration results are compared as shown in Table 2.

[0051] reaction system

[0052] It can be seen from the test results that although the product concentration of the immobilized lactic acid bacteria beads of the present invention is slightly lower than that of the unimmobilized bacteria liquid when used for the first time, it can be used repeatedly and can maintain the reactivity.

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Abstract

The invention refers to a process for using yeasting method to produce lactic acid. Takes out the mixed liquid of embedding agent and fungus suspending liquid to solidify the cells, the embedding agent is made up of fungus suspending liquid: 2% kala glue liquid: 8-12% polyvinyl alcohol and 2-4 sodium alginate with proportion 1:1:3-5, combines with using pH controller to control the acid rate pH value = 5.0-6.2 automatically, when the pH gauge displays the pH value is 5.0 in the yeasting liquid, the pH control system switches on the power automatically, the circular pump begin to work, when the pH value reaches 6.5, the relay cuts off the power, the circular pump stops, the yeasting is going on, it realizes in situ separation yeast lactic acid generating.

Description

technical field [0001] The technical solution claimed in the present invention relates to the preparation method of lactic acid, specifically the process of producing lactic acid by fermentation. Background technique [0002] Many of biofermentation and biocatalysis are product-inhibiting processes. If the products are not neutralized or separated in time, the fermentation process will not continue. Lactic acid fermentation is a typical product-inhibiting fermentation process. The suitable acidity for lactic acid fermentation is pH value=5.0~6.2, when the pH value is less than 5, the fermentation will be inhibited and the yield of lactic acid will decrease. During the lactic acid fermentation process, due to the accumulation of lactic acid, the pH value of the fermentation broth gradually decreased, which seriously inhibited the growth of lactic acid bacteria and the synthesis of lactic acid. When using the traditional fermentation method to produce lactic acid, in order t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C12P7/56
Inventor 马建芳李剑徐子钧王仁静孙雪莲李明智刘如林
Owner TIANJIN GUARD T & D
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