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High-frequency test tube plant regeneration and industrial seedling growing of smoke tree

A test-tube plant and high-frequency technology, applied in the field of culture medium, can solve the problems of mixed good and bad, low reproductive efficiency, and no available female parent, and achieve the effect of uniform seedlings, high reproductive efficiency, and pure species

Inactive Publication Date: 2004-09-08
BEIJING BAOSHENGZHUANG PLANT SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not only do these propagation methods require a large number of "mother" trees, they are also very inefficient
In addition, due to the successful introduction and domestication of American red oak in my country, the red oak ("Chinese red oak" or "Beijing red oak") found in my country is still in the stage of individual plant observation, and there are not a large number of female parents available, and the market demand and large enough to cause some to reproduce by seed sowing
The genetic variation of seed seedlings is very large, good and bad are intermingled, and it is difficult to maintain their excellent horticultural traits

Method used

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  • High-frequency test tube plant regeneration and industrial seedling growing of smoke tree

Examples

Experimental program
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Effect test

Embodiment 1

[0012] Example 1: Surface disinfection of explants

[0013] The pure species of Rhizoma sinica was used as the female parent tree for explants. Collect young shoots, new shoots and semi-lignified 1-year-old shoots as explants. After the shoots are removed, rinse them with tap water for 30 minutes, and rinse the new shoots and semi-lignified 1-year-old shoots with tap water for 30-60 minutes, cut into 3 -5cm stem segments. The washed buds and stems are soaked in 70-75% alcohol for 10-15s, then transferred to 3-5% sodium hypochlorite solution for 5, 10, 15 or 30 minutes, or transferred to 1-2% arsenic-mercury solution Medium treatment for 5, 10, 15 or 20 minutes, or after disinfection by sodium hypochlorite, transfer to arsenic and mercury solution for further disinfection. Immediately after surface disinfection treatment, rinse with sterile water 5 times for at least 5 minutes each time. The surface-sterilized young shoots and 1-year-old semi-lignified shoot explants were cu...

Embodiment 2

[0030] Example 2: Selection of explants and minimal medium

[0031] There are 7 kinds of basic medium tested: MS (Murachige and Skoog, 1962), B5 (Gomborg et al., 1968), WPM (Woody Plant Medium, Lloyd and McCrown, 1980 ), Lescure (Lescure, 1966), MS-B5 (MS inorganic salts and B5 organics), WPM5 (WPM inorganic salts and B5 organics), and L5 (Lescure 5 inorganic salts and B5 organics). For callus induction and bud differentiation, the sucrose concentration in the above basic medium is 3%, and the agar is 0.7% (or 7g / L). After the basic medium is prepared, add BA 5.0mg / L and 2,4-D 2.0mg / L, adjust the pH to 5.6-5.8, divide into bottles or autoclave and then divide into petri dishes, each bottle is about 60ml, 25-30ml per Petri dish. The medium was autoclaved at 125°C for 20-25min.

[0032] Three kinds of surface-sterilized explants (tender shoots, young shoots and semi-lignified shoots) were inoculated into the above medium, placed in the dark at 25±2°C for cultivation, and by...

Embodiment 3

[0048] Example 3: Effect of Plant Growth Regulator (PGR) on Callus Induction

[0049] According to the result of embodiment 1 and 2, use tender shoot as explant, take WPM5 as basic medium, have studied cytokinin BA, 2IP, KT or ZT (0,0.5,1.0,2.0 or 5.0, unit: mg / L, the same below) alone and in combination with auxin 2,4-D, IAA, IBA or NAA (0, 0.5, 1.0, 2.0, 3.0 or 5.0) on callus induction. The preparation of the culture medium was carried out according to the method of Example 2, and the disinfection of the explants was carried out according to the method of Example 1. The inoculation method of the explants was carried out according to the method in Example 2.

[0050] The cultures were grown in the dark for 2 weeks, then switched to light. 16 hours of light and 8 hours of darkness every day, the light intensity is about 1000-1500Lx. The culture temperature is 25±1°C.

[0051] Observation and statistics after 4 weeks of culture, in the presence of 2,4-D (0.5, 1.0, 2.0 or 5...

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Abstract

The present invention provides the technology of high-frequency test tube plant regeneration and industrial seedling growing of smoke tree. The technology includes selecting explant; surface sterilizing explant; inducing callus; high frequency differentiation and regeneration of seedling; fast growing of regenerated seedling; fast propagation of regenerated seedling in test tube; high frequency rooting of regenerated seedling in test tube; and transplanting the regenerated plant to soil and domestication. The whole process may be completed within 40 days, and the present invention has fast propagation rate as high as 1,000,000 one year. The seedling is clonic, pure and regular.

Description

technical field [0001] The invention relates to the technique of utilizing biotechnology to realize the high-frequency test-tube plant regeneration, rapid propagation and factory seedling production of red cotinus sinensis and the culture medium used therein. Background technique [0002] Red sumac (Cotinus coggygria var. cinerea Engl.et Wils) is a variant of Cotinus coggygria (Cotinus Mill.) in the Anacardiaceae family (Anacardiaceae), and it is also considered to be a red leaf variant of Cotinus coggygria (Van der Zwaan AG , Publicatie, -Landbouw-Economisch-Instituut, -Netherlands.1989, 4(122): 30), also known as "red leaves", "smoke tree", English name "Ash-coloured Smoketree" or "Purpureus", is a large Deciduous shrubs or small trees. Because red oak was first discovered in the United States, it is also collectively called "American red oak". Some people also named it according to the place where it was found, such as "European cotinus", "Chinese cotinus" or "Beijing c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 肖兴国肖胜涛肖应涛
Owner BEIJING BAOSHENGZHUANG PLANT SCI TECH
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