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Oral staphylococcus aureus filtrate preparation for improving immune function

A staphylococcus and body immunity technology, which is applied in the fields of bacterial medical raw materials, medical preparations containing active ingredients, blood diseases, etc., can solve the problem of lack of oral biological preparations and other problems, achieve less toxic and side effects, improve activity, and curative effect exact effect

Inactive Publication Date: 2004-07-07
SHENYANG XIEHE GRP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the lack of current oral biological preparations, the object of the present invention is to provide a convenient oral Staphylococcus aureus filtrate preparation for improving the body's immune function

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Inoculate 0165 strains on 1ml of LB slant, culture at 37°C for 12 hours, pick a single colony, transfer to a conical flask containing 100ml of LB culture medium, and cultivate in a constant temperature shaker at 30°C for 8 hours until logarithmic growth period, according to 5% (by volume percentage) inoculation amount, inoculated in the fermenter, then cultivated at 35 ° C for 20 hours, centrifuged to remove the obtained fermented liquid after the bacterial cells were concentrated, and the obtained sample was passed through the ion chromatography column (Using carboxymethyl cellulose in this embodiment) purification; use 5mmol / L PB buffer (PH5.5) and 20mmol / L PB buffer (PH6.8) to elute respectively, collect the required components, and prepare , adding aspartame, sterilizing, and dispensing.

Embodiment 2

[0023] Inoculate 0165 strains in 1ml of LB culture medium, culture on a shaker at 37°C for 12 hours, then transfer to a conical flask containing 30ml of LB culture medium, and cultivate on a constant temperature shaker at 30°C for 8 hours until the logarithmic growth phase, According to the inoculum amount of 5% (by volume percentage), inoculate in the effective tank, and then cultivate at 30°C for 20 hours; the filtrate obtained after centrifuging to remove the bacteria is dialyzed and concentrated, and the obtained sample is passed through an ion chromatography column. (Using carboxymethyl cellulose in this embodiment) purification; use 8mmol / L PB buffer (PH5.0), 10mmol / L PB buffer (PH6.8), 45mmol / L PB buffer (PH6.5) respectively ) step-by-step elution, collect the required components, prepare, add aspartame, sterilize, and pack.

experiment example

[0025] This experiment uses Laca mice, male, weighing 18-20g. The experimental group is first treated with cyclophosphamide, and then given high (360ng / Kg), medium (180ng / Kg) and low (90ng / Kg) doses (medicine of the present invention), and give Yunzhi polysaccharide, adopt intragastric administration, once a day, each administration volume is 0.5ml / 20g, continuous administration 10 days, put to death animal and carry out each item in 24 hours after drug withdrawal. Check, the experimental results are shown in Table 1 below.

[0026] group

Spleen weight (g)

spleen index

(ng / g)

P value

normal control group

0.272±0.05

9.22±2.03

＜0.01

high dose group

0.230±0.06

8.97±2.60

＜0.01

Middle dose group

0.21±0.04

7.96±1.69

＜0.05

low dose group

0.200±0.05

7.44±1.32

＜0.05

Yunzhi polysaccharide group

0.220±0.04

8.84±1.47

＜0.01

cyclophosphamide group

0...

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Abstract

An orally applied filtrate of staphylococcus aureus for improving immunity and increasing the number of white cells and white platelets in human body contains enterotoxin SEA, SEB, SEC, SED and TSST-1. Its advantages are sure curative effect and low toxic by-effect.

Description

technical field [0001] The invention relates to biomedicine, in particular to an oral staphylococcus aureus filtrate preparation for improving the body's immune function. Background technique [0002] Nowadays, people communicate more and more frequently, and diseases caused by bacteria and viruses are also taking advantage of the opportunity. Especially when people's immune function is low, they are vulnerable to invasion and disease, which affects their health. Children and the elderly are more susceptible to infection due to their weak immune function. Infestation by germs. [0003] Tumor is one of the diseases that seriously threaten human life and health. At present, it has become the biggest killer of human beings; from the perspective of current treatment methods, there are mainly two methods, chemotherapy and radiotherapy, among which chemotherapy drugs have a greater effect on normal cells. Toxic and side effects seriously affect the immune function of the body. W...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K38/16A61P7/00A61P37/04
Inventor 陈巨余
Owner SHENYANG XIEHE GRP LTD
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