Determination of one section vagina bacillus Gardner DNA sequence (3) specificity and its application
A technology of Gardnerella and DNA sequence is applied in the field of determination of DNA sequence specificity of Gardnerella vaginalis, and the effect of low cost and simple operation is achieved.
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[0019] First, using Gardnerella vaginalis (Gardnerella vaginalis) as the keyword, the nucleotide sequence of Gardnerella vaginalis (Gardnerella vaginalis) was searched in the gene database. A total of several closely related nucleotide sequences were obtained, and these nucleotide sequences were compared with the nucleotide sequences (nr) in the gene database using the Blast online software on the NCBI network to exclude non-specific nucleotide sequences, A 1 Kb specific nucleotide sequence was obtained. More than 20 pairs of PCR primers were designed based on this sequence, DNA was extracted from clinical specimens, PCR amplification was performed, and a pair of sequences and primers suitable for PCR amplification were screened out. The products obtained by PCR amplification were consistent with the expected size (see Fig. 1. No. 1 in the figure is standard molecular weight DNA, and the molecular weights from top to bottom are: 622, 527, 404, 309, 242 / 238, 217, 201, 190, 180,...
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