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Submerged culturing method for making mushroom liquid bacterial and culture medium therefor

A technology of deep fermentation culture and liquid culture medium, which is applied to the deep fermentation culture method of Lentinus edodes liquid strain and the culture medium field thereof, can solve problems such as shortening culture time, and achieve the effects of shortening culture time, vigorous growth and strong mycelium.

Inactive Publication Date: 2003-12-31
BEIJING JINXIU DADI AGRI CO LTD
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Problems solved by technology

[0003] The present invention overcomes the deficiencies of the existing methods for fermenting and cultivating liquid strains of shiitake mushrooms, and designs a submerged fermentation and culturing method for liquid strains of shiitake mushrooms that can cultivate strains of uniform and moderate size and shorten the cultivation time

Method used

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  • Submerged culturing method for making mushroom liquid bacterial and culture medium therefor

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Embodiment 1

[0021] Embodiment 1: the cultivation method of mushroom liquid bacterial classification: present embodiment is preferred embodiment, and its concrete steps are: (1), female species is cultivated in female species culture medium: get 0.5cm 2 See square shiitake mushroom mother species, inoculate it into a special mother species culture medium, and culture it in the dark at 23±1°C for 7 days to activate and subculture the strains to form a test tube mother species; the special mother species medium for this step The prepared content and ingredients are: 100 parts of special mother seed medium contains 20 parts of potato, 1.5 parts of shiitake mushroom waste, 2 parts of glucose, 0.05 part of yeast extract, 0.1 part of peptone, KH 2 PO 4 : 0.1 part, MgSO 4 ·7H 2 O: 0.05 parts, 2 parts of agar, water: 76.2 parts, the pH value of the medium obtained by the above components is 6.0; and the original potato medium, yeast extract is 0.25 parts, and there are 5 parts of wheat bran, thi...

Embodiment 2

[0024] Embodiment 2: the liquid culture medium among the embodiment 1 adopts original mushroom liquid culture medium, and its composition and content are: contain 20 parts of potatoes, 2 parts of glucose in 100 minutes of liquid culture medium, (NH 3 ) SO 4 : 0.2 parts, KH 2 PO 4 : 0.1 part, MgSO 4 ·7H 2 O: 0.05 parts, vitamin B1: 0.001 parts, agar 0.05 parts, and the rest is water. Do not contain cornflour and starch in this formula, but contain potato and glucose (monosaccharide), therefore relative embodiment 1 cost is higher. Other steps and conditions of this embodiment are the same as embodiment 1.

Embodiment 3

[0025] Embodiment 3: the composition and content of special-purpose female seed medium can also be: 15 parts of potatoes in 100 parts of special-purpose female seed medium, 3 parts of shiitake mushroom waste, 1 part of glucose, 0.02 part of yeast extract, 0.2 part of peptone, KH 2 PO 4 : 0.2 parts, MgSO 4 ·7H 2 O: 0.07 parts, 2 parts of agar, and the rest are water.

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Abstract

The present invention provides one kind of deep fermentation process of culturing liquid Xianggu mushroom seed and its culture medium, and aims at providing one liquid Xianggu mushroom seed process superior to available technology. The culture process includes the steps of: mother seed culture in mother seed culturing medium; dark transitional culture of the obtained mycelium in sawdust culture medium at 22-27 deg.c for 12-20 days; the first stage of shaking culture of the crushed sawdust seed inside liquid culture medium for 5-7 days; the second stage of shaking amplification culture in fresh liquid culture medium; and fermentation treatment of the shaking cultured mushroom liquid to obtain liquid Xianggu mushroom seed product. The relevant culture medium includes culture medium for Xianggu mushroom mother seed, sawdust culture medium and deep fermenting culture medium.

Description

Technical field: [0001] The invention relates to a method for fermenting and cultivating strains, in particular to a method for submerging fermenting and cultivating liquid strains of shiitake mushrooms and a culture medium thereof. Background technique: [0002] The production of shiitake mushrooms has always used solid strains for production. In recent years, some people have begun to carry out research on the fermentation and cultivation technology of liquid strains, but there are still many problems in this strain used for production. (1) For the reproduction of the mother species, use more General-purpose culture medium, there is no culture medium specially used for shiitake mushroom strains, so the breeding time of the mother species is longer, and the quality of mycelia is not high; (2) The inoculation of the production liquid strains is mostly cultivated with the mother species culture medium Bacteria directly carries out one-level shake flask culture, and the time o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14C12N1/22
Inventor 林翔鹰党俊梅刘富段新梅王玉忠李荣旗
Owner BEIJING JINXIU DADI AGRI CO LTD
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