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Camellia chrysantha culture medium for tissue culture and quick breeding

A technology of tissue culture rapid propagation and medium, applied in the direction of angiosperms/flowering plants, plant cells, botanical equipment and methods, etc., can solve the problems of unsatisfactory effects of Camellia japonica species

Inactive Publication Date: 2003-12-10
GUANGXI FUXIN SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The publication number is 85102805, which is called the invention patent of Camellia japonica tissue culture medium, which discloses an improved medium based on ER, but it has only been applied experimentally on C. pingguoensis D. fang , according to research, it does have an effect on the tissue culture application of Pingguo Camellia, but it is not ideal for Camellia species that are distributed in high-temperature and rainy tropical rainforest ecology and account for the vast majority of Camellia species and varieties.
In addition, there is no report on other Camellia japonica tissue culture rapid propagation medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] formula:

[0024] NH 4 NO 3 1650mg / L KNO 3 1900mg / L

[0025] Ca(NO 3 ) 2 .4H 2 O300mg / L MgSO 4 .7H 2 O 370mg / L

[0026] K H 2 PO 4 340mg / L KI 0.83mg / L

[0027] H3BO 3 0.63 MnSO 4 .4H 2 O 22.3mg / L

[0028] ZnSO 4 .7H 2 O 8.6mg / L Na 2 Mo 4 .H 2 O 0.025mg / L

[0029] CuSO 4 .5H 2 O 0.025mg / L CoCl 2 .6H 2 O 0.0025mg / L

[0030] FeSO 4 .7H 2 O 27.8mg / L Na 2 .EDTA.7H 2 O 37.3mg / L

[0031] Inositol 50mg / L Niacin 0.5mg / L

[0032] Pyridoxine Hydrochloride 0.5mg / L Thiamine Hydrochloride 0.5mg / L

[0033] Glycine 2mg / L Sucrose 2-4%

[0034] Agar 6.5g / L CaCl 2 2H 2 o

[0035] Indole-3-acetic acid 0.5mg / L 3-indolebutyric acid 0.5-mg / L

[0036] 6-Benzylaminopurine 3mg / L Activated carbon 0.4g / L

[0037] Types of camellia

Embodiment 2

[0039] Adjust the formula as follows:

[0040] NH 4 NO 3 1650mg / L KNO 3 1900mg / L

[0041] Ca(NO 3 ) 2 .4H 2 O 300mg / L MgSO 4 .7H 2 O 370mg / L

[0042] K H 2 PO 4 340mg / L KI 0.83mg / L

[0043] H3BO 3 0.63 MnSO4 .4H 2 O 22.3mg / L

[0044] ZnSO 4 .7H 2 O 8.6mg / L Na 2 Mo 4 .H 2 O 0.025mg / L

[0045] CuSO 4 .5H 2 O 0.025mg / L CoCl 2 .6H 2 O 0.0025mg / L

[0046] FeSO 4 .7H 2 O 27.8mg / L Na 2 .EDTA.7H 2 O 37.3mg / L

[0047] Inositol 50mg / L Niacin 0.5mg / L

[0048] Pyridoxine Hydrochloride 0.5mg / L Thiamine Hydrochloride 0.5mg / L

[0049] Glycine 2mg / L Sucrose 2-4%

[0050] Agar 6.5g / L

[0051] 3-Indolebutyric acid 3mg / L

[0052] Activated carbon 0.8g / L 6-benzylaminopurine 0.1mg / L

[0053] Types of camellia

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Abstract

The present invention uses improved MS culture medium as basis, and adds the active carbon, indole-3-acetic acid, 3-indolebutyric acid and 6-benzylaminopurine as auxiliary agent to make tissue cultivation of two key stages of induced growth of bunch bud and seedling-strengthening and rooting of camllia chrysantha. The invention can be used for implementing industrial quick seedling cultivation.

Description

1. Technical field [0001] The invention relates to a tissue culture and rapid propagation medium for camellia japonica, in particular to a tissue culture and rapid propagation medium for the two key stages of camellia camellia cluster bud induction and strong seedling rooting. 2. Background technology [0002] Camellia (C. chysantha) is a plant of Camellia sect. nitidisima Chang (Camellia sect. nitidisimaChang), and there are 28 species and varieties recorded so far. Golden Camellia is known as the "Giant Panda of the Plant Kingdom" and "Queen of the Tea Clan". Because of its extremely high ecological requirements for survival, the natural stock is small, and it is mainly distributed in individual areas in southern my country. It is a rare species. In order to protect this rare species, people have carried out scientific research on resource protection and artificial propagation of Camellia japonica. At present, the artificial propagation of camellia camellia mainly adopts ...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01H4/00A01H5/00C12N5/04
Inventor 秦绪雄
Owner GUANGXI FUXIN SCI & TECH
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