Breeding method of humi grass using low energy heavy ion radiation mutagenesis

Abstract

A mutagenic method with low-energy heavy ion radiation for culturing the seeds of Humi herb used to build the grazing land in shoal and nunja includes such steps as low-energy heavy ion radiation to the embryonic calli of Humi herb to lead and mutation of genetic material, differentiating to generate regenerated seedlings, growing in artificial greenhouse, screening excellent line by testing its characteristics including protein content, and comparing DNA variation to obtain a new variety.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for selecting and breeding high-quality new strains of Spartina viticum by utilizing low-energy heavy ion radiation mutagenesis. 2. Background technology [0002] Spartina patens is a high-quality salt-tolerant forage grass, and it is also a common tool species for salinity restoration. There are a large number of bare tidal flats in the eastern coastal areas of my country, which lack suitable vegetation. The introduction and development of high-quality new strains of Spartina fox in this type of tidal flats is of great significance to the development of saline soil agriculture and restoration of tidal flats in my country. [0003] The introduction of Spartina fox in China began in the 1980s. There is a single domestic species, and there is no report on its genetic breeding. In the past, the reports of ion beam radiation mutation breeding were all focused on crop seeds an...

AI Technical Summary

Problems solved by technology

However, none of the work involved the genetic improvement of Spartina foxii and the screening of new excellent strains, and the tissue culture techniques used were complex and wasteful of reagents