Serum-free medium of human epidermis cell and its cultivating method and application

Abstract

A non-serum culture medium for human epidermal cells is based on IMDM culture medium and features additional use of hydrogen peroxidase, bovin serum albumin, human transferrin, cholesterin, insulin, hydrocortisone, epidermal cell growth factor, ox hypophysis extract and ethanolamine. Its advantages are no interference and high correctness and precision. It can be used for medicine research and medical purpose.

Description

technical field [0001] The invention relates to a cell culture medium and its culture method and application, in particular to a human epidermal cell serum-free medium and its application. Background technique [0002] The in vitro expansion and culture of human epidermal cells plays an important role in burn treatment, dermatology and pharmacology research. The traditional culture of epidermal cells is to add a certain proportion of serum to the culture medium. However, the composition of serum is very complex, with many unknown factors, and the growth factors and hormones contained in different batches of serum vary greatly. Therefore, traditional serum cultures affect the precise study of epidermal cell growth regulation mechanisms. Since the 1980s, foreign scholars have devoted themselves to exploring the possibility of serum-free culture of epidermal cells and achieved a certain degree of success, but they have not yet reached the growth rate of in vitro culture method...

AI Technical Summary

Problems solved by technology

The traditional culture of epidermal cells is to add a certain proportion of serum to the culture medium. However, the composition of serum is very complex, there are many unknown factors, and the growth factors and hormones contained in each batch of serum vary greatly.

Therefore, the precise study of the mechanism of growth regulation of epidermal cells has been influenced by traditional serum culture.

Since the 1980s, foreign scholars have devoted themselves to exploring the possibility of serum-free culture of epidermal cells and achieved a certain degree of success, but they have not yet reached the growth rate of in vitro culture methods with serum.