Rose extract and its application

A rose flower extract and extract technology, applied in the field of rose flower extract, can solve problems such as no reports, and achieve the effects of abundant plant resources, efficient removal of superoxide anions, and easy extraction

Inactive Publication Date: 2003-04-16
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, extracting antioxidant active substances from roses has not been reported so far

Method used

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  • Rose extract and its application
  • Rose extract and its application
  • Rose extract and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Rose flower extract (F 21 ) preparation and purification

[0020] Collect the plant roses produced in Fujian, chop the dried plant flowers, soak them in water, and boil them under reflux for 2 hours. , manufactured by Beijing Sihuan Scientific Instrument Factory).

[0021] Take 50mg of dry powder and dissolve it in an appropriate amount of ammonium acetate buffer (10mM, pH4.5), load it on a CM-32 cation exchange cellulose chromatography column, and then use 10mM pH4.5, 200mM pH4.5, 400mM pH8. 0 ammonium acetate buffer solution to elute sequentially, automatically collect step by step, and measure the antioxidant activity of each tube.

[0022] The separated active substance was applied to a Sephadex G-75 chromatographic column, collected by elution with phosphate buffer (50 mM, pH 7.0), and freeze-dried.

[0023] The composition of the extract is determined

[0024] f 21 Three kinds of active ingredients F were separated through the above-mentioned CM-32 cation exc...

Embodiment 2

[0064] Select SAM mice (provided by Tianjin Disease Prevention Center) male, 8 months old as experimental animals to group, 6 in the administration group, 6 in the control group, rose extract (F 21 ) was added with water to make an appropriate aqueous solution, administered by intragastric administration for 30 days, the dosage was 80 mg / kg bw, and the control group was given normal saline at the same dose, once a day, and the mice in each group were taken from the liver at the same time on the 31st day. 10% liver tissue homogenate (w / v) was prepared from the tissue, and the supernatant was taken after refrigerated centrifugation. The supernatant was diluted 100 times in volume to obtain 0.1% interstitial fluid. The method of total SOD activity determination is as follows:

[0065] Reagent Assay tube (ml) Control tube (ml)

[0066] PBS 1.0 1.0

[0067] Tissue homogenate supernatant 0.03

[0068] Distilled water 0.5 0.5+0.03

[0069] ...

Embodiment 3

[0078] The selection, grouping, administration and administration time of the mice were the same as in Example 2. After 30 days of administration, the experimental mice were killed by neck dislocation, and the liver tissue was taken to make 10% liver homogenate. After centrifugation, 1ml of the supernatant was taken, and 10μl of absolute ethanol was added. 100 110μl, ice bath for 5 minutes. Dilute the sample 200 times and add to the reaction system, add 2ml of enzyme solution to the assay tube, mix with 1ml of 10mM PBS, measure the change of absorbance at 240nm for 30 seconds, and calculate the CAT enzyme activity.

[0079] The average CAT enzyme activity of the 6 mice in the treatment group was 491.01K / g.Hb, and the average CAT enzyme activity of the 6 mice in the control group was 361.20K / g.Hb. The average activity of the CAT enzyme in the treatment group was 129.81K higher than that of the control group / g.Hb.

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Abstract

An extract of Rosa rugosa Thunb is disclosed, and contains rose extract (30%) which contains polyose (84.74%), protein (8.57%) and gallic acid derivative (6.69%). It plays such roles as suppressing erythrocyte haematolysis by 100% and peroxidization of tissue lipid by more than 90% and increasing the activity of antioxidant enzyme. It can be used to prepare antioxidant for preventing and treatingdiseases and increasing immunity.

Description

Technical field [0001] The invention relates to the extraction of natural plants, especially the rose extract. The extract has natural antioxidant activity and can be made into an antioxidant for preventing and treating diseases, improving immunity of the body, and as a health care product for prolonging life. Background technique [0002] Judging from the current research results, oxygen free radicals are not only related to aging, but also to most of the common major human diseases, from cardiovascular and cerebrovascular diseases with the highest human mortality rate to the most terrifying cancers and AIDS in humans, all of which are consistent with Free radicals are closely related. By preventing the generation of free radicals and removing existing free radicals, the purpose of disease prevention and treatment is achieved. At present, the artificially synthesized antioxidants have certain toxicity and mutagenicity, and their reuse is greatly restricted. Therefore, rese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P39/06
Inventor 刘方牛淑敏吴子斌
Owner NANKAI UNIV
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