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Polypeptide-human transferring factor concerned with anti-withering protein-16.94 and polynucleotide for encoding it

An anti-apoptotic protein and polynucleotide technology, applied to peptide/protein components, anti-animal/human immunoglobulins, peptide sources, etc.

Inactive Publication Date: 2003-04-02
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there is evidence that E1B 19K can affect gene expression, but whether this is due to its anti-apoptotic function is unknown

Method used

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  • Polypeptide-human transferring factor concerned with anti-withering protein-16.94 and polynucleotide for encoding it
  • Polypeptide-human transferring factor concerned with anti-withering protein-16.94 and polynucleotide for encoding it
  • Polypeptide-human transferring factor concerned with anti-withering protein-16.94 and polynucleotide for encoding it

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Experimental program
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Effect test

Embodiment 1

[0120] Total RNA was extracted from human fetal brain by one-step method of guanidine isothiocyanate / phenol / chloroform. Poly(A) mRNA was isolated from total RNA using Quik mRNA Isolation Kit (product of Qiegene). 2ug poly(A) mRNA was reverse transcribed to form cDNA. Smart cDNA cloning kit (purchased from Clontech) was used to insert the cDNA fragment into the multiple cloning site of the pBSK(+) vector (product of Clontech Company), transform DH5α, and the bacteria formed a cDNA library. The sequences of the 5' and 3' ends of all clones were determined using Dyeterminate cycle reaction sequencing kit (product of Perkin-Elmer) and ABI 377 automatic sequencer (Perkin-Elmer). Comparing the determined cDNA sequence with the existing public DNA sequence database (Genebank), it was found that the cDNA sequence of one of the clones, 3053c03, was a new DNA. The insert cDNA fragment contained in this clone was determined bidirectionally by synthesizing a series of primers. The resu...

Embodiment 2

[0121] The human anti-apoptotic protein-related transcription factor-16.94 sequence of the present invention and its encoded protein sequence were used by the Blast program (Basiclocal Alignment search tool) [Altschul, SF et al.J.Mol.Biol.1990; 215: 403-10], and performed homology searches in databases such as Genbank and Swissport. The gene with the highest homology to the human anti-apoptotic protein-16.94-related transcription factor of the present invention is a known transcription factor related to the mouse anti-apoptotic protein Bcl-2, and the protein encoded by it has the access number of Genbank for AF249273. The results of protein homology are shown in Figure 1. The two are highly homologous, with an identity of 93% and a similarity of 96%. Example 3: Cloning of the gene encoding human anti-apoptotic protein-related transcription factor-16.94 by RT-PCR

Embodiment 3

[0121] The human anti-apoptotic protein-related transcription factor-16.94 sequence of the present invention and its encoded protein sequence were used by the Blast program (Basiclocal Alignment search tool) [Altschul, SF et al.J.Mol.Biol.1990; 215: 403-10], and performed homology searches in databases such as Genbank and Swissport. The gene with the highest homology to the human anti-apoptotic protein-16.94-related transcription factor of the present invention is a known transcription factor related to the mouse anti-apoptotic protein Bcl-2, and the protein encoded by it has the access number of Genbank for AF249273. The results of protein homology are shown in Figure 1. The two are highly homologous, with an identity of 93% and a similarity of 96%. Example 3: Cloning of the gene encoding human anti-apoptotic protein-related transcription factor-16.94 by RT-PCR

[0122] The total RNA of fetal brain cells was used as a template, and oligo-dT was used as a primer to carry out...

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Abstract

A polypeptide-human anti-wither protein associated transcription factor-16.94, the polynucleotide for coding, the process for preparing said polypeptide by DNA recombination, the application of said polypeptide in treating diseases such as tumor, immunopathy, etc, the antagonist of said polypeptide and its medical action, and the application of said polynucleotide are disclosed.

Description

technical field [0001] The invention belongs to the field of biotechnology. Specifically, the invention describes a new polypeptide-human anti-apoptosis protein-related transcription factor-16.94, and a polynucleotide sequence encoding the polypeptide. The present invention also relates to the preparation method and application of the polynucleotide and polypeptide. Background technique [0002] The adenovirus E1B 19K protein is a potent inhibitor of apoptosis that acts in conjunction with E1A to deform primary rodent cells. E1B 19K shares sequence and functional homology to the mammalian anti-apoptotic gene product Bcl-2. Like Bcl-2, E1B 19K can bind to and antagonize several cell death proteins including Bax, Bak, and Nbk / Bik. In addition, there is evidence that E1B 19K can affect gene expression, but whether this is due to its anti-apoptotic function is unknown. [0003] In 1995, Becky A. Speelman et al. used the yeast two-hybrid system to per...

Claims

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Application Information

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IPC IPC(8): C07H21/04C07K14/435C07K14/47C07K16/18C12N15/10C12N15/11C12N15/113C12N15/12C12N15/36C12N15/64
Inventor 毛裕民谢毅
Owner FUDAN UNIV
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