Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Engineered scaffold of amniotic membrane tissue and process for removing cells from aniotic membrane

A technology of tissue engineering scaffolds and amniotic epithelial cells, which can be applied to artificial cell constructs, tissue culture, animal cells, etc., can solve the problems of inability to transplant into the body, inability to use tissue engineering scaffolds, and inability to plant other cells, etc., to achieve a good society The effect of benefit, low cost and simple method

Inactive Publication Date: 2002-09-18
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amniotic membrane has no blood supply, the basement membrane layer and the dense layer have no cellular components, and has good ductility and high mechanical strength. It is a natural tissue engineering scaffold, but because one side of the amniotic membrane is covered by columnar epithelial cells, and the other side is embedded with fibroblasts, it cannot be planted. cell
Tseng et al. (2001) planted fibroblasts in the surface layer of the sea. Since the opposite side still had epithelial cells, they could not be transplanted into the body, so they could not be used as tissue engineering scaffolds.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] How to remove the epithelial cell layer and fibroblasts of the amnion is the key to the use of the amnion as a tissue engineering scaffold material, and is also a key problem to be solved by the present invention.

[0016] For example, the amnion with the epithelial cells removed from the epithelial layer can be obtained by decellularizing the amnion by the following method:

[0017] (1) Soak the amniotic membrane with 0.2% trypsin solution at 37°C for 25 minutes, or take any combination in the range of 0.2-5% trypsin solution for 20-30 minutes;

[0018] (2) Fix the amniotic membrane, stir and rinse during or after soaking, wash away the epithelial cells of the amniotic membrane epithelial cell layer and the fibroblasts of the fibroblast layer, if the washing is not complete, scrape off the remaining with a cell scraper superficial cells.

[0019] The amnion can be obtained by decellularizing the amnion with the following method:

[0020] (1) Use 4.5% trypsin solution...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An engineered scaffold of tissue is prepared from the amniotic membrane whose epicytes and fibrous matericytes has been removed. A process for removing said cells from amniotic membrane includes immersing the amniotic membrane in 0.2-5% trypsase solution at 37 deg.C for 20-25 min and stirring while flushing. Its advantages are no immunoreaction and simple process.

Description

technical field [0001] The invention relates to the field of tissue engineering for manufacturing human organs and tissues, in particular to an amniotic membrane tissue engineering scaffold used as a tissue engineering scaffold and an amniotic membrane decellularization method. Background technique [0002] Tissue engineering research is the science of manufacturing human organs and tissues. Due to its broad application prospects and increasing industrial benefits year by year, governments and industries of various countries have invested heavily in research and industrial construction. The world's annual research funding is more than billions of dollars. my country's 863,973, climbing plan has also invested several hundred million yuan in research. Tissue engineering research includes two main aspects: one is the scaffold used to build organs and tissue shapes; the other is the cells that can grow and play physiological functions on the scaffold. For tissue engineering sc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/00C12N5/071
Inventor 雪原
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com