Biopreparation of textiles of high temp.

A technology for fabrics and compounds, applied in the field of cotton fabrics, can solve the problems of inactivity and limit the biological scouring of fabrics, and achieve the effects of short processing time, effective emulsification and dewaxing, and complete compatibility.

Inactive Publication Date: 2002-03-27
NOVOZYMES NORTH AMERICA INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, all reported bacterial pectinases require divalent cations for their activity and are generally inactive at temperatures above 60°C.
Because (i) fabrics must be pre-boiled to thin the waxy skin covering the pectin layer, and (ii) calcium ions tend to form insoluble salts and precipitate on the fiber surface, these characteristics limit their application to fabric bioscouring

Method used

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  • Biopreparation of textiles of high temp.
  • Biopreparation of textiles of high temp.

Examples

Experimental program
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Embodiment 2

[0025] It is understood that any polypeptide exhibiting the properties described above may be used in the practice of the present invention. In other words, pectate lyases derived from other organisms can be used as long as the polypeptides produced exhibit the above-mentioned high-temperature activity (and preferably, pH-optimum and divalent cation-independence of the activity), or derived from the above-mentioned enzymes by adding, Pectate lyases derived from deletion or substitution of one or more amino acids, including hybrid polypeptides. Such pectate lyase variants useful in the practice of the present invention can be generated using conventional mutagenesis procedures and identified using, for example, high throughput screening techniques such as the agar plate screening procedure described in Example 1 below.

[0026] The temperature, pH, and divalent cation dependencies of isolating pectate lyase can be determined using conventional methods. For example, an enzyme a...

Embodiment Embodiment 1

[0060] EXAMPLES Example 1: Determination of the properties of thermostable pectate lyases

[0061] The following method was used for qualitative analysis of pectate lyase activity. 1. Pectate lyase assay

[0062] For this experiment, a 0.1% sodium polygalacturonate (Sigma P-1879) solution was prepared in 0.1 M glycine buffer (pH 10). Take 4ml of this solution and preheat at 40°C for 5 minutes. Then, add 250 μl of enzyme (or enzyme diluent), mix the reaction system on the mixer at the highest speed for 10 seconds, and incubate at 40°C or other temperatures for 20 minutes, then measure the absorbance at 235nm: 0.5ml cuvettes, absorbance at 235nm was measured continuously on a HP diode array spectrophotometer in a temperature-controlled cuvette holder. For steady state, a linear increase of at least 200 seconds was used to calculate the rate.

[0063] For the calculation of the catalytic rate, an increase of 5.2A235 / min corresponds to the formation of 1 μ...

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Abstract

The present invention provides methods for high-temperature biopreparation of cellulosic fibers by contacting the fibers with pectin-degrading enzymes, preferably thermostable, alkaline, divalent cation-independent pectate lyases, under conditions compatible with scouring and bleaching technologies.

Description

field of invention [0001] The present invention relates to a process for the biofabrication of cellulose fibers, preferably fabrics, most preferably cotton fabrics, at elevated temperatures using a thermostable pectate lyase. Background of the invention [0002] Removal of non-cellulosic components found in natural fibers, as well as removal of impurities, such as compounds added to the fibers, such as sizing and lubricants used in processing machinery, are important aspects of making fabrics from cellulosic fibers. Removal of non-cellulosic impurities, known as "scouring," ideally produces fabrics with uniform high wettability, allowing for uniform bleaching and / or dyeing. [0003] Traditional scouring processes typically employ highly alkaline chemical treatments that not only remove impurities but also weaken the underlying cellulose content of the fibers or fabrics. In addition, chemical refining presents environmental concerns for wastewater treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12S3/04D06L4/40D06M16/00D06M101/04
CPCD06M16/003D06L3/11D06L4/40D06M16/00
Inventor L·康斯巴克M·舒莱恩P·A·胡赛因N·E·K·朗格M·伯卓恩瓦德
Owner NOVOZYMES NORTH AMERICA INC
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