Reverse protein chip

Abstract

The present indention relates to the reverse protein chip and its preparation and detection in life science field and may be used widely in biology, medicine and related domain. In the reverse protein chip, tested protein, polypeptide or other biomolecule is fixed on solid carrier through chemical bond connection before the solid carrier is enclosed. Labeled fluorescent probe is added and made toproduce hybridization reaction with solid biomolecule, and after eluting uncombined matter, the fluorescent strength in sample point is proportional directly with the concentration of tested component. The present invention can perform multiple sample detection simultaneously.

Description

technical field [0001] The invention relates to a reverse protein chip and its preparation and detection. Protein chips belong to the field of life sciences. A protein chip is an array of proteins or polypeptides arranged in an orderly manner on a solid substrate to achieve parallel detection, identification, identification and diagnosis of proteins. Widely used in biology, medicine and related fields. Background technique [0002] The protein chip patent we applied for before, the protein chip for immunoassay (application number: 01105795.5), the main technical route is: spot the probe corresponding to the protein to be tested on the solid substrate, and then add the sample to be tested and the probe Hybridization, adding fluorescent markers, and finally judging the concentration of a certain component to be tested in the sample to be tested according to the fluorescence intensity. This method is used for the analysis and detection of antigens, antibodies or ligands, and...

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Problems solved by technology

[0003] However, first, the number of samples that can be detected by this method is limited. If it is used in a multi-sample microarray biochip, since each chamber can only detect one sample, the above technique is only suitable for several to dozens of samples detection

If there are too many chambers, the consequences are: first, the distance between

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