Fish meal proteolytic products, its preparation process and application

A protein hydrolyzate and fish meal technology, applied in the field of protein hydrolyzate, can solve the problem of less protein hydrolyzate

Inactive Publication Date: 2007-02-14
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing protein hydrolyzate with herbicidal activity is relatively small, to provide a kind of fishmeal protein hydrolyzate which can inhibit the germination of weed seeds and its preparation method. Herbicides that Inhibit Germination of Grass Seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] After adding deionized water to the fish meal, shake it in a constant temperature oscillator at 60°C. After the temperature of the solution in the triangular flask is kept at a constant temperature, adjust the pH of the solution to 8.0 with 20% ammonia water to become the substrate. The mass ratio of fish meal: deionized water = 1 : 9; add alkaline protease to the substrate for reaction, shake continuously during the reaction, keep the temperature at 60 ℃, and add 20% ammonia water dropwise to maintain the pH value of the reaction system at 8.0, the reaction time is 6h, according to the mass ratio to the bottom substance:alkaline protease=1:2; the reaction solution was placed in a 100°C water bath and heated for 15min to inactivate the enzyme, then centrifuged at 4000r / min for 20min, and the supernatant was frozen and dried to dry powder. Refrigerate for later use.

[0017] The fish meal used is the FAQ fish meal imported from Peru, and the raw materials of the fish mea...

Embodiment 2

[0028] Similar to Example 1, the difference is that after adding deionized water to the fish meal, it is shaken in a constant temperature oscillator at 60 ° C. After the temperature of the solution in the triangular flask is kept constant, the pH of the solution is adjusted to 7.8 with 20% ammonia water to become the substrate. The mass ratio of fish meal: deionized water = 1:8; alkaline protease was added to the substrate for reaction, and during the reaction, the temperature was kept at 60 ° C, and 20% ammonia water was added dropwise to maintain the pH value of the reaction system at 7.8. The reaction time was 5h, and the mass ratio of substrate:alkaline protease=1:1; the reaction solution was placed in a 100°C water bath and heated for 10min to inactivate the enzyme, then centrifuged at 3800r / min for 15min, and the supernatant was frozen and dried into Dry powder, refrigerated for later use.

Embodiment 3

[0030] Similar to Example 1, the difference is that after adding deionized water to the fish meal, it is shaken in a constant temperature oscillator at 60 ° C. After the temperature of the solution in the triangular flask is kept at a constant temperature, the pH of the solution is adjusted to 8.5 with 20% ammonia water to become the substrate. Press The mass ratio of fish meal: deionized water = 1:10; alkaline protease was added to the substrate for reaction, and the temperature was kept at 60°C during the reaction, and 20% ammonia water was added dropwise to maintain the pH value of the reaction system at 8.5. The reaction time was 8 h, and the mass ratio of substrate: alkaline protease = 1:3; the reaction solution was placed in a 100 °C water bath and heated for 20 min to inactivate the enzyme, then centrifuged at 4200 r / min for 25 min, and the supernatant was frozen and dried to form a solution. Dry powder, refrigerated for later use.

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Abstract

Provided are fish meal proteolytic products, its preparation process and application, wherein the preparing process comprises, charging deionized water into fish meal, stirring in a container, charging ammonia to adjust the pH of the solution, charging basified protease for reaction, then charging ammonia to maintain the pH of the reaction system, water-bathing the reaction solution, removing enzyme, centrifuging, freeze drying the supernatant fluid into dried powder, finally freezing for later use.

Description

technical field [0001] The invention relates to a protein hydrolyzate, in particular to a hydrolyzate prepared by using animal feed fish meal protein and a preparation method and its application in the pre-emergence control of weeds. Background technique [0002] Herbicides have been widely used in weed control in farmland, orchards and urban lawns. There are many types of herbicides, but most of them are chemical herbicides produced by chemical synthesis. Although chemical herbicides can control the growth and spread of weeds to a limited extent, the problem of their pollution to soil and groundwater is becoming increasingly apparent. Therefore, the production of natural herbicides without toxic and side effects is an inevitable trend in the development of herbicides. In recent years, some natural active peptides and proteolytic peptides in some organisms have been found to have herbicidal activity, which provides a new idea for innovative work on how to develop herbicides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/04A23J3/30A01N63/02
Inventor 杨剑卢昌义于兴娜叶勇
Owner XIAMEN UNIV
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