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Composition and method for regulating cell proliferation and cell death

A composition and bladder technology, applied in the directions of drug combinations, carbohydrate active ingredients, medical raw materials derived from bacteria, etc., can solve problems such as toxicity, side effects, drug resistance or development of immune sensitization.

Inactive Publication Date: 2001-04-04
贝尔尼奇泌尿知识产权公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many of these agents are ineffective or toxic, have significant side effects, lead to the development of resistance or immune sensitization, and debilitate recipients

Method used

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  • Composition and method for regulating cell proliferation and cell death
  • Composition and method for regulating cell proliferation and cell death
  • Composition and method for regulating cell proliferation and cell death

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Preparation of MCC from Mycobacterium phlei and Purification of M-DNA from MCC and Mycobacterium phlei

[0059] MCC was prepared from Mycobacterium phlei (110 strains), M-DNA was purified from MCC (MCC-DNA), M-DNA was purified from Mycobacterium phlei (Mycobacterium phlei- DNA). All reagents are selected to enhance DNA preservation. Unless otherwise stated, MCC, MCC-DNA, and M. phlei-DNA were resuspended in DNase-free water or a pharmaceutically acceptable DNase-free buffer by Sonicate for emulsification. MCC, MCC-DNA and M. phlei-DNA were free of endotoxin as determined using the Limulus Amoeba-like Cell Lysate QCL-1000 Kit (BioWhittaker, Walkersville, MD).

Embodiment 2

[0061] Preparation of bacterial-DNA-bacterial cell wall complexes and bacterial DNA from other bacterial species

[0062] As described in Example 1, from Mycobacterium smegmatis, Mycobacterium fortuitum, Nocardia rubra (Nocardia rubra), Nocardia asteroides (Nocardia asteroides), Corynebacterium parvum, Kansas Mycobacterium, M. tuberculosis and M. bovis produce bacterial DNA-bacterial cell wall complex (BCC) and bacteria-DNA (B-DNA).

Embodiment 3

[0064] DNase treatment

[0065] At 25°C, in 20mM Tris HCl, pH 8.4, 2mM MgCl 2 and 50 mM KCl, MCC-DNA containing 1 μg of M-DNA and MCC and Regressin® (US Patent 4744984) were digested with 1 International Unit (IU) of RNase-free DNase I (Life Technologies) for 1 Hour. The method of inactivating DNase I was to add EDTA to a final concentration of 2.5 mM and heat at 65°C for 10 minutes.

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Abstract

The present invention relates to a composition and method for treating cancer in the urinary bladder. More particularly, the present invention relates to a composition comprising a Mycobacterium phlei (M. phlei) deoxyribonucleic acid (M-DNA)-M. phlei cell wall complex (MCC), wherein the M-DNA is preserved and complexed on the M. phlei cell wall, and a pharmaceutically acceptable carrier. The MCC composition inhibits proliferation of and induces apoptosis in the cancer cells in the urinary bladder of the animal.

Description

field of invention [0001] This application incorporates by reference US Provisional Application 60 / 075111, filed February 18, 1998, which is claimed in its entirety. [0002] The present invention comprises a mycobacterial deoxyribonucleic acid (B-DNA)-mycobacterial cell wall complex (BCC), wherein the B-DNA is preserved and complexed on the bacterial cell wall, enabling the BCC to be effective in the treatment of bladder cancer . More specifically, the present invention comprises a Mycobacterium Phlei (M. phpei)-DNA (M-DNA)-Mycobacterium Phlei cell wall complex (MCC), wherein the M-DNA is preserved and Compounded on the cell wall of Mycobacterium phlei, MCC can effectively inhibit the proliferation of bladder cancer cells and induce cell apoptosis. Background of the invention [0003] Cancer is an abnormal net accumulation of atypical cells that can result from hyperproliferation, inadequate apoptosis, or a combination of both. Apoptosis is initiated by ligand binding to...

Claims

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Application Information

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IPC IPC(8): A61K31/7088A61K31/70A61K35/74A61K47/48A61P13/10A61P35/00
CPCA61K47/48776A61K31/711A61K31/713A61K47/6901A61P13/10A61P35/00
Inventor 奈杰尔·C·菲利普斯马里翁·C·菲利翁
Owner 贝尔尼奇泌尿知识产权公司
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