Foot-and-mouth disease virus antigen dilution technology

Abstract

A technique for diluting the antigen of foot-and-mouth disease virus uses a diluent prepared from sodium chloride, disodium hydrogen phosphate, potassium chloride, potassium dihydrogen phosphate, magnesium sulfate, glucose and calcium chloride through proportional mixing and concentrating by 10 times. Before it is used, its pH value must be regulated to 7.6 by 7.5% NaHCO3. Its advantages are simple and reliable method and low cost.

Description

(1) Technical field [0001] The invention belongs to the technical field of dilution of veterinary biological products, in particular to a dilution technology of foot-and-mouth disease virus antigen. (2) Background technology [0002] Foot-and-mouth disease is one of the most important animal diseases in the world. It is listed as the first class A disease by the International Office of Epizootics (OIE). . In order to effectively control and prevent foot-and-mouth disease, some countries that have eliminated or are controlling foot-and-mouth disease have established regional joint defense or their own national foot-and-mouth disease vaccine / antigen bank. Although our country successfully developed pig and cattle foot-and-mouth disease O-type inactivated vaccine in the late 1980s and used it for actual epidemic prevention, its quality and quantity are far from meeting the needs of actual epidemic prevention, and it has not yet established its own national vaccine / antigen bank...

AI Technical Summary

Problems solved by technology

So far, there is no special technology in this area in China. In related research, 0.5% hydrolyzed milk protein solution is generally used to dilute the FMD virus antigen. Although this method is simple, it does not affect the infectivity and 140s antigen content of FMD virus. Relatively speaking, the cost is high, and it cannot meet the requirements of large-scale industrialized production of foot-and-mouth disease vaccines, nor does it meet the national conditions of our country.

Countries that have established FMD regional defense or their own national vaccine / antigen banks such as Canada, Mexico, the United States, Australia, New Zealand, Ireland, the United Kingdom, Norway, Sweden, the Netherlands, Finland, Germany, France, Italy, Thailand, FAO In the establishment and application of the FMD vaccine / antigen library, the Yatai Regional Animal Production and Health Committee mainly diluted the preserved virus antigen with phosphate buffered saline (PBS), but it has been proved by experiments that PBS can reduce the infection of FMD virus titer, thus affecting the immune efficacy of the vaccine, and has a certain toxic effect on experimental animals-suckling mice