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Method and apparatus for separating shaped compositions from composition of complex biological liquid sample

A technology for biological liquids and liquid samples, which can be used in sampling devices, biological material analysis, preparation of test samples, etc., and can solve problems such as filter cost and filter clogging

Inactive Publication Date: 2005-10-05
斯蒂芬·C·沃德罗 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Problems encountered with this technique include the cost of the various filters, the need to know the size of the target forming component, the tubing required to force the sample through the filter, and the possibility of filter clogging

Method used

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  • Method and apparatus for separating shaped compositions from composition of complex biological liquid sample
  • Method and apparatus for separating shaped compositions from composition of complex biological liquid sample
  • Method and apparatus for separating shaped compositions from composition of complex biological liquid sample

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Embodiment Construction

[0029] Now refer to the attached drawings. FIG. 1 is a schematic diagram of a sample container, which is represented by the number 2 as a whole. The container 2 includes a chamber 4 defined by a side wall 6 and a flat bottom wall 8. On the bottom wall 8 of the chamber 4, an equal-thickness layer made of a hydrogel 9 which is preferably transparent or translucent is provided. A suitable hydrogel includes the "PHYTA" gel, which is a hydrogel formed of hexaerythritol, rhamnose, and glucose. It is transparent and colorless, so it is a good material for inspection of forming components. This hydrogel is a product of Sigma Diagnostics.

[0030] Other suitable hydrogels include: polyethylene oxide, poly(ethylene oxide and propylene oxide copolymer), poly(vinylpyrrolidone), poly(vinyl alcohol), poly(acrylamide), poly(vinyl acetate), poly (Acrylic) (in Na + In the form of), poly (copolymer of acrylic acid and acrylamide) (in Na + In the form of), poly(acrylic acid) (in Na + In the form of)...

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Abstract

Formed constituents in an aqueous based fluid biologic material sample are separated from the aqueous constituent of the sample, and are concentrated in an examining instrument's focal plane where they can be examined under magnification. Examples of fluids that can be analyzed in this fashion include urine; cerebrospinal fluid; pleural fluid; ascites; fluids aspirated from cysts such as thyroid and breast cysts; cytologic specimens which have been placed in an aqueous fluid; platelet-rich plasma; and the like. The sample is placed in a chamber having a layer of a hydrophilic hydrogel covering a surface of the chamber. An opposite surface of the chamber is transparent, and may be formed by a microscope slide cover slip, or the like. The volume of hydrogel in the chamber is sufficient so that, when the hydrogel absorbs essentially all of the aqueous fraction of the sample, the hydrogel will expand and fill the chamber. The capture surface of the expanded hydrogel is preferably planar, and any formed constituents in the sample will be captured on the capture surface of the hydrogel layer, and will not be absorbed into the hydrogel. Formed constituents, such as: cells; bacteria; crystals; protozoa; ova; parasites; and the like, can be differentially highlighted by use of labeled antibodies, selective stains, or the like, so as to enable optical examination and differentiation of various formed constituents which may be in the sample. Formed constituents may be harvested from the capture surface of the expanded hydrogel layer for more detailed examination and analysis. The capture surface of the hydrogel may be provided with a plurality of beads for use in locating the capture surface with an optical scanning instrument, and for re-establishing previously scanned fields of view.

Description

Technical field [0001] The present invention relates to a device and method for separating a shaped component from a liquid component of a biological liquid sample so as to facilitate the study of the shaped component with an optical instrument. More specifically, the present invention relates to a device and method that produces a shaped component and places the component on a plane of the device that coincides with the focal plane of the optical instrument. Background technique [0002] The shaped components in complex biological liquid samples are usually isolated or separated from the liquid components of the sample so that the shaped components can be carefully studied. Flow blood count is a technique used to identify formed components like blood cells in a blood sample. Using this technology, various types of blood cells and other formed components in the blood can be distinguished from each other and can be counted. During this procedure, the blood sample must be diluted b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/483B01L3/00G01N1/10G01N1/28G01N33/48G01N33/49G02B21/34
CPCG01N33/491Y10T436/2575Y10T436/25G02B21/34B01L3/508
Inventor 斯蒂芬·C·沃德罗
Owner 斯蒂芬·C·沃德罗
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