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Process for preparing hypericum perforatum extract

A technology of Hypericum perforatum and extract, which is applied in the field of preparation of Hypericum perforatum extract, which can solve the problems of failing to meet quality requirements and ignoring value

Inactive Publication Date: 2005-08-17
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This extract does not meet the quality requirements of China's national second-class new drug raw materials, and also ignores the value of active ingredients such as hypericin and flavonoids in the extract

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]Take 1100g of Hypericum perforatum medicinal material powder, extract it with acetone for 3 times at room temperature under constant stirring, and the amount of solvent used is 4000ml, 3000ml, 2500ml in sequence. Filter and combine the extracts to obtain 7800ml of extracts. After adding 2300ml of distilled water to the extract, extract with 5000ml of petroleum ether each time for a total of 2 extractions, and combine the extracts. Take the acetone aqueous layer at 50°C to recover acetone under reduced pressure, acidify the aqueous phase with 10% hydrochloric acid to pH = 2, let stand at 4°C for 48 hours, centrifuge, wash the precipitate with water until it is neutral, and freeze-dry to obtain 8.68 g of brown precipitate. Concentrate the petroleum ether phase to 2200ml, extract with 3000ml 50% ethanol, and discard the alcoholic aqueous phase. Extract the petroleum ether phase with 50% ethanol solution containing 3% NaOH, acidify to pH=2 with 10% hydrochloric acid, extrac...

Embodiment 2

[0025] Get Hypericum perforatum medical material powder 400g, with 1% composite stabilizer (V C :V E : citric acid 100: 50: 1) at room temperature and leaching 3 times under constant stirring, the solvent consumption is respectively 2000ml, 1500ml, 1500ml. Filter and combine the extracts to obtain 4200ml of extracts. After adding 1500ml of distilled water to the extract, extract with 1000ml of n-hexane each time for a total of 2 extractions, and combine the extracts. Take the acetone aqueous layer at 50°C to recover acetone under reduced pressure, acidify the aqueous phase with 10% hydrochloric acid to pH = 0, let it stand at 4°C for 72 hours, centrifuge, wash the precipitate with water until it is neutral, and then dry it in vacuum to obtain dark brown extract B.84g. Add 0.5% vitamin E to the n-hexane phase, concentrate to 800ml, extract twice with 600ml 50% ethanol, and discard the alcoholic aqueous phase. with 5% NaHCO 3 60% ethanol solution to extract petroleum ether p...

Embodiment 3

[0027] Get Hypericum perforatum medical material powder 500g, with 3% stabilizing agent (V C Palmitate: V E : 95% ethanol of benzoic acid 200: 20: 1) leaching 3 times under room temperature and constantly stirring, and solvent consumption is respectively 2500ml, 1800ml, 1800ml. Filter and combine the extracts to obtain 5200ml of extracts. After adding 1800ml of distilled water to the extract, extract with 980ml of diethyl ether each time for a total of 2 extractions, and combine the extracts. The alcoholic water layer was recovered under reduced pressure at 70°C to recover ethanol, the aqueous phase was acidified to pH=0 with 10% hydrochloric acid, allowed to stand at 4°C for 72 hours, centrifuged, the precipitate was washed with water until neutral, and then freeze-dried to obtain 4.56 g of a dark brown extract. Vitamin E was added to the ether phase to a content of 1%, extracted twice with 600ml of 50% ethanol, and the alcoholic aqueous phase was discarded. Extract the et...

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Abstract

The present invention relates to the preparation process of extract containing three active components including hypericin, Hyperforin and flavones in the total content not less than 50% from hypericum perforatum or other congeneric plant. The three active components are separated from the polar solvent extracted liquid via biphase extraction with non-polar solvent selected based on their difference in polarity. They are then purified separately based on their difference in acid-base property to obtain the extract A with high hypericin and flavones content and extract B with high Hyperforin content. The two extracts are merged through dissolution to obtain hypericum perforatum extract with total active component content not less than 50%. The extract has the obvious activity of resisting depression and may be used as the material of depression treating medicine or functional food.

Description

technical field [0001] The invention relates to a method for preparing an extract containing hypericin, hypericin and flavonoids from Hypericum perforatum or other plants of the same genus, and the total content of the active components is more than or equal to 50%. Background technique [0002] Hypericum perforatum (also known as Hypericum perforatum) Hypericum perforatum L. is a plant of the genus Hypericum in the family Garcinia. Hypericin, hyperforin and flavonoids are the main active ingredients or medicinal ingredients for antidepressant or anti-anxiety. Hypericum perforatum extract has been clinically used in the treatment of moderate and mild depression in Germany. [0003] Hypericin has antiviral, antitumor, antidepressant and other pharmacological effects. Molecular weight 504.43, blue-black needle-like crystal (pyridine-methanol containing HCl), melting point 320°C (decomposition), easily soluble in pyridine or other organic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C46/10C07C50/36
Inventor 杨得坡王发松黄亚东胡海燕
Owner SUN YAT SEN UNIV
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