Mineralized collagen material, preparation method and application

A collagen material and collagen technology, applied in medical science, prosthesis and other directions, can solve the problems of high industrialization cost, poor mineralization uniformity, complicated preparation steps, etc., and achieve the effect of excellent flexibility and good elasticity

Active Publication Date: 2022-08-02
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of this application is to address the deficiencies in the prior art, to provide a mineralized collagen material, preparation method and application, to at least solve the problems of poor mineralization uniformity, complicated preparation steps, low efficiency, and inability to mass-produce in the related art. The problem of high production and industrialization costs

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  • Mineralized collagen material, preparation method and application
  • Mineralized collagen material, preparation method and application
  • Mineralized collagen material, preparation method and application

Examples

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Embodiment 1

[0068] The present embodiment is an exemplary embodiment of the present invention, and relates to a mineralized collagen material, a preparation method and an application thereof.

[0069] A preparation method of a mineralized collagen material, comprising:

[0070] Step S102, dissolving type I collagen, calcium source and acid in a first solvent to obtain a first solution, wherein the first solvent is glycerol or a mixed solvent of glycerol and water;

[0071] Step S104, dissolving the alkali and the phosphorus source in a second solvent to obtain a second solution, wherein the second solvent is glycerol or water or a mixed solvent of glycerol and water;

[0072] Step S106, when the temperature is less than or equal to 45°C, adding the second solution to the first solution and mixing to obtain a third solution, wherein the pH of the third solution is greater than or equal to 7;

[0073] Step S108, soaking the third solution in a deionized water solvent or an ethanol solvent ...

Embodiment 2

[0131] This embodiment is a specific embodiment of the present invention.

[0132] Add calcium chloride, porcine-derived collagen and acetic acid to the glycerol / water mixture (the mass ratio of glycerol and water is 2:3) at room temperature under stirring with a magnetic stirring bar (rotation speed is 500-600 rpm / min) to prepare A first solution containing 0.06 M calcium chloride, 0.1 M acetic acid and 15 mg / mL collagen was prepared.

[0133] Trisodium phosphate and sodium hydroxide were dissolved in glycerol to prepare a second solution containing 0.05M trisodium phosphate and 0.5M sodium hydroxide.

[0134] 3.2 mL of the second solution was slowly dropped into 4.6 mL of the first solution at 10° C. under stirring with a magnetic stirring bar (800-1000 rpm), and mixed thoroughly to obtain the third solution.

[0135] The third solution was placed at room temperature, and the contents were taken out after 1 day, immersed in deionized water repeatedly, and freeze-dried to pr...

Embodiment 3

[0137] This embodiment is a specific embodiment of the present invention.

[0138] Add calcium chloride, mouse-derived collagen and acetic acid into deionized water at room temperature under stirring with a magnetic stirring bar (rotation speed 500-600 rpm) to prepare a mixture containing 0.1M calcium chloride, 0.1M acetic acid and 5mg / mL The first solution of collagen.

[0139] Trisodium phosphate and sodium hydroxide were dissolved in glycerol to prepare a second solution containing 0.05M trisodium phosphate and 0.5M sodium hydroxide.

[0140] 3.2 mL of the second solution was slowly dropped into 5.0 mL of the first solution at 10° C. under stirring with a magnetic stirring bar (800-1000 rpm), and mixed thoroughly to obtain the third solution.

[0141] The third solution was placed at room temperature, and the contents were taken out after 2 days, immersed in deionized water repeatedly, and freeze-dried to prepare a mineralized collagen scaffold.

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Abstract

The invention relates to a mineralized collagen material, a preparation method and application, and the preparation method comprises the following steps: dissolving type I collagen, a calcium source and acid in a first solvent to obtain a first solution; dissolving alkali and a phosphorus source in a second solvent to obtain a second solution; adding the second solution into the first solution under the condition that the temperature is less than or equal to 45 DEG C, and mixing to obtain a third solution; and soaking the third solution in a deionized water solvent or an ethanol solvent, and removing impurities to obtain the mineralized collagen gel. The method has the advantages that glycerol is introduced into a reaction system, so that the assembly speed and the assembly structure of collagen can be controlled, the nucleation and crystallization speed and the structure of mineral substances can be controlled, the collagen can be assembled into a large-size space network structure at a controllable speed, meanwhile, mineralization is deeply carried out, and continuous large-size mineralized collagen blocks are prepared; the collagen can be highly mineralized in a very small reaction system, and the collagen can be prepared without repeatedly replacing a mineralizing solution, a macromolecular additive and extra cross-linking.

Description

technical field [0001] The invention relates to the technical field of material synthesis, in particular to a mineralized collagen material, a preparation method and an application. Background technique [0002] A considerable part of bone defects caused by trauma, infection, tumor, etc. cannot be repaired by themselves. Bone transplantation and bioengineering materials are the main means to solve this problem. Although bone transplantation, especially autologous bone transplantation, is the "gold standard" for the treatment of bone defects, it is the "gold standard" for the treatment of bone defects. Problems limit the further popularization of bone grafting techniques. [0003] In contrast, bioengineered materials can well compensate for the deficiencies of bone grafting techniques. Among the many bioengineered materials, mineralized collagen has become the most popular in the field of bone regeneration due to its good biocompatibility, bioactivity, osteopromoting abilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/02A61L27/12A61L27/50A61L27/52A61L27/54A61L27/56
CPCA61L27/24A61L27/025A61L27/12A61L27/50A61L27/52A61L27/54A61L27/56A61L2430/02A61L2300/412A61L2300/252A61L2300/112
Inventor 陈峰赵云飞路丙强赵新宇贺石生
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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