Application of protein OsGATA6 in regulating and controlling flowering time, spike type, grain shape and thousand grain weight of plant
A technology of flowering time and protein, applied in angiosperms/flowering plants, botanical equipment and methods, applications, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0084] Example 1. Acquisition of OsGATA6 gene
[0085] The inventors of the present invention have cloned a new GATA transcription factor OsGATA6 gene (LOC_Os01g54210) from rice after extensive experiments. Sequence analysis showed that the OsGATA6 gene contained two exons of 291 bp and 873 bp in size, encoding a protein composed of 387 amino acids.
[0086] The full-length nucleotide sequence of the OsGATA6 gene is shown in SEQ ID NO.1.
[0087] The nucleotide sequence of the coding region of the OsGATA6 gene is shown in SEQ ID NO.2.
[0088]The protein encoded by the OsGATA6 gene is named as protein OsGATA6, and its amino acid sequence is shown in SEQ ID NO.3.
Embodiment 2
[0089] Example 2. Application of protein OsGATA6 in regulating rice flowering time, panicle shape, grain shape and thousand-grain weight 1. Construction of recombinant plasmid
[0090] 1. Construction of recombinant plasmid pUN1301-OsGATA6
[0091] (1) Using the genomic DNA of rice cultivar ZH11 as a template, a primer pair consisting of OsGATA6OX-F and OsGATA6OX-R was used to perform PCR amplification to obtain PCR amplification products.
[0092] (2) The PCR amplification product was double digested with restriction enzymes BamHI and KpnI, and the digested product was recovered.
[0093] (3) The vector pUN1301 (Li D, Wang L, Wang M, XuYY, Luo W, Liu YJ, Xu ZH, Li J, Chong K (2009). Engineering OsBAK1 gene was digested with restriction enzymes BamHI and KpnI. As amolecular tool to improve rice architecture for high yield. Plant Biotechnol J. 7(8):791-806.), the vector backbone was recovered.
[0094] (4) Connect the enzyme digestion product recovered in step (2) and the vec...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com