Antifreeze protein-containing diluent for improving preservation quality of chicken semen and preparation method thereof

A diluent and cryopreservation technology, which is applied in the biological field, can solve the problems of inability to provide long-term preservation, and achieve the effects of reducing oxidative damage, easy-to-obtain ingredients, and low toxicity

Active Publication Date: 2022-07-29
贵州省种畜禽种质测定中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Does not provide long-term storage in frozen environments such as liquid nitrogen

Method used

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  • Antifreeze protein-containing diluent for improving preservation quality of chicken semen and preparation method thereof
  • Antifreeze protein-containing diluent for improving preservation quality of chicken semen and preparation method thereof
  • Antifreeze protein-containing diluent for improving preservation quality of chicken semen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Construction of chicken semen cryopreservation system

[0026] The configuration of chicken semen cryopreservation diluent according to the following components

[0027]Configuration of pine needle antifreeze polypeptide solution: Dilute the pine needle antifreeze polypeptide shown in SEQ ID NO.1 in PBS buffer (0.01M pH7.4), and adjust the concentration to 1mg / ml;

[0028] The configuration of liquid I: fructose 5~10g, trehalose 8~20g, potassium citrate monohydrate 1~3g, magnesium acetate tetrahydrate 4~6g, sodium glutamate 11~19g, Tris (tris (trihydroxymethylaminomethane)) 8~13g, reduced glutathione 0.1~0.5g, oxidized glutathione 0.05~0.1g, dissolved in 1000ml of water, fully dissolved, then add 1~5ml of pine needle antifreeze polypeptide liquid as I liquid, use 0.22 Filter and sterilize with a um filter, and dispense into 2ml centrifuge tubes for storage;

[0029] Configuration of liquid II: take 660ml of liquid I, add 80~120ml of DMA, it is liquid II, fil...

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Abstract

The invention provides a diluent for cryopreservation of chicken semen. The diluent for cryopreservation of chicken semen is characterized by comprising pine needle antifreeze polypeptide, fructose, trehalose, potassium citrate monohydrate, magnesium acetate tetrahydrate, sodium glutamate, Tris (trihydroxymethyl aminomethane), reduced glutathione, oxidized glutathione and dimethylacetamide (DMA). The chicken semen cryopreservation diluent has the advantages that the raw materials are easy to obtain, the preparation is convenient, the fertilization rate reaches 80% or above, and the DNA integrity is higher. Compared with related data of fresh chicken essence, DNA integrity of the chicken essence is close to that of fresh essence, sperm motility and hatching rate of the chicken essence are slightly lower than those of the fresh essence, and the chicken essence has a wide application prospect.

Description

technical field [0001] The invention relates to the field of biology, relates to a diluent containing antifreeze protein for improving the preservation quality of chicken semen and a preparation method thereof, and further relates to the application of the diluent in the cryopreservation of chicken semen. Background technique [0002] Semen cryopreservation is a major innovation in artificial insemination technology. It solves the problem of long-term preservation of semen, so that semen is not limited by time, region and life of breeding animals, and can make full use of the genetic performance of the best stage of fine breeds, maximize and improve the utilization rate of fine breed cocks, and greatly improve the genetic progress to accelerate the pace of breeding and improvement of varieties. At the same time, it is of great significance and application value to determine the progeny of elite roosters in a short period of time, to retain and restore the excellent characte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 周迪蒋桂荣杨蓉王燕赵忠海唐明艳王府刘虓任丽群袁勇谭晓山敖叶
Owner 贵州省种畜禽种质测定中心
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