Resistance evaluation method for potato late blight

Abstract

The invention relates to a potato late blight resistance evaluation method. The method comprises the following steps: 1) identifying physiological races of potato late blight bacteria; (2) inoculating a potato late blight bacterium spore suspension; 3) investigating potato late blight, wherein the morbidity is equal to diseased plant number / total plant number * 100%; 4) establishing a potato late blight nursery; and 5) evaluating the field resistance of the potato late blight: evaluating the disease resistance on the basis of the area AUDPC under a disease development curve, expressing the AUDPC value by using the percentage value of the potato susceptibility rate in a period of time, converting the corrected relative AUDPC value CrAUDPC from the AUDPC, and judging the infection type by using the CrAUDPC value as an evaluation basis according to a formula. According to the method, a systematic and scientific potato late blight field resistance evaluation method is formed, and the identification result has comparability, reliability and stability.

Description

technical field [0001] The invention relates to the technical field of bacterial wilt resistance identification, in particular to a resistance evaluation method of potato late blight. Background technique [0002] Potato is the fourth largest food crop in my country. Potato late blight caused by Phytophthora infestans is one of the important bottlenecks restricting the development of my country's potato industry. For a long time, the breeding and utilization of disease-resistant varieties has been the most economical and effective strategy to control late blight. The breeding and promotion of potato disease-resistant varieties are actively carried out in China, which have broad application prospects in production. In the process of disease resistance breeding, it is usually necessary to establish a stable and accurate identification method for disease resistance, so that the breeding materials for disease resistance can be accurately selected. Establishing a disease nurser...

AI Technical Summary

Problems solved by technology

At the same time, in actual production, it is found that different varieties have different levels of resistance to late blight, and the disease resistance of varieties also changes with the passage of planting years. There is a lack of scientific operating specifications on how to carry out identification of disease resistance of varieties In this regard, combined with the epidemic characteristics of potato late blight, continuous follow-up investigations have been carried out to form a practical and effective resistance evaluation method

[0004] The occurrence and prevalence of potato late blight are closely related to meteorological conditions such as temperature and humidity. Not every field can be affected. At present, disease nurseries are mainly selected in areas with high incidence of potato late blight and the occurrence of the disease cannot be fully guaranteed. For this reason, an accurate Potato late blight resistance identification nursery with high stability and high stability; at the same time, potato late blight is a typical multi-cycle airborne disease. stage, it is necessary to form a set of systematic and scientific field disease resistance evaluation methods so that the results are comparable, reliable and stable

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