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Botryosphaeria botryoides HN17496 strain, biocontrol inoculant and preparation method and application of biocontrol inoculant

A technology of HN17496, a bio-control agent, applied in the field of bio-control, can solve the problems such as being unsuitable for the bio-control of Helminthium umbilicalus, environmental hazards, etc., and achieve significant bio-control effects, good bio-control effects, and no bio-control effects. The effect of security risks

Pending Publication Date: 2022-07-22
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the present stage, the control methods of Helminoptera barley root rot are mainly soaking seeds with chemicals, or spraying chemical agents, both of which are harmful to the environment. In 2010, a strain of bacteria, Stenotrophomonas maltophilia C3, was discovered. This strain can produce chitin-degrading enzymes and has a good control effect on Umbilophilia rot. However, this bacterium is an opportunistic pathogenic bacterium, and is not suitable as a biocontrol bacterium for Umbilicola solani
However, there are seldom reports on the biocontrol fungi against Helminthes rot in wheat roots

Method used

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  • Botryosphaeria botryoides HN17496 strain, biocontrol inoculant and preparation method and application of biocontrol inoculant
  • Botryosphaeria botryoides HN17496 strain, biocontrol inoculant and preparation method and application of biocontrol inoculant
  • Botryosphaeria botryoides HN17496 strain, biocontrol inoculant and preparation method and application of biocontrol inoculant

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preparation example Construction

[0035] The present invention also provides the preparation method of the above-mentioned biocontrol agent, comprising the following steps:

[0036] The above-mentioned HN17496 strain is inoculated into a PDA medium, and cultured for 5-7 days to obtain the biocontrol agent.

[0037] In the present invention, the temperature of the culture is preferably 25 to 28°C.

[0038] In the present invention, when the biocontrol agent does not contain HN17496 strain, after culturing for 5-7 days, sterilization treatment is preferably included; the sterilization treatment method preferably includes: sterilization at 121° C. for 30 minutes.

[0039] The present invention also provides the application of the above HN17496 strain or the above biocontrol fungicide or the biocontrol fungicide prepared by the above preparation method in preventing and treating plant diseases, wherein the pathogenic bacteria of the plant diseases include Helminthosporium rhizogenes. In the present invention, the...

Embodiment 1

[0042] From the soil under the weeds in Thrushi Valley, Lushan County, Pingdingshan, the HN17496 strain of Staphylococcus aureus was obtained by the dilution plate method. The specific method is as follows:

[0043] Weigh 10 g of soil, add it to a conical flask containing 90 mL of sterilized water, place the conical flask on a shaker at 120 rpm, and shake for 25 min to make soil particles evenly dispersed in distilled water to obtain a soil suspension with a dilution factor of 10; Draw 1 mL of the suspension and put it into a test tube containing 9 mL of sterilized water to obtain a suspension with a dilution factor of 100;

[0044] The WA medium was sterilized at high temperature and cooled to 45°C, added streptomycin 30 μg / mL, 4 drops of chloramphenicol, poured into a petri dish to solidify and cooled down, and the obtained soil suspension with a dilution factor of 100 was fully shaken. Aspirate 1 mL, drop 5 drops into each petri dish, spread it evenly with a sterilized curv...

Embodiment 2

[0054] Identification of rescreened strains

[0055] The HN17496 bacterial strain of the rescreening of Example 1 was inoculated on the PDA medium, cultivated for 7d at 25°C, to obtain a bacterium colony with a diameter of 2.2cm, and the morphological characteristics of the bacterium colony were as follows:

[0056] Colonies are black, sporogenous stems growing on medium, viscous, covered with gray to black sporozoites, dark brown on the back of the petri dish. No ascus. The hyphae will produce dark brown secretions, like impurities, often in bundles, forming hyphal bridges. Conidia giant linear, solitary or aggregated growth, thin-walled, branched or unbranched, erect or slightly curved, 1 to 2 septa, smooth, conidiophores 18.2 to 64.0 μm long, meristematic The width of sporophore is 2.8~4.9μm, and 1~6 sporogenous cells are attached to the top. The sporogenous cells are phialoid, cudgel-like or cudgel-like, transparent, translucent or dark black, with a smooth surface, 6.6...

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Abstract

The invention relates to the technical field of biocontrol bacteria, in particular to an ear botryosphaeria HN17496 strain, a biocontrol microbial inoculum as well as a preparation method and application of the biocontrol microbial inoculum. The preservation number of the strain HN17496 disclosed by the invention is CCTCC (China Center For Type Culture Collection) NO: M2022489. The grape ear mold HN17496 strain provided by the invention has a very good biocontrol effect on wheat root rot and bihilum, and has no potential safety hazard to people and livestock; according to the embodiment of the invention, the inhibition rate of the ear mold HN17496 strain and the metabolite of the ear mold HN17496 strain on wheat root rot bihilum spore is 65%-72%, and the biocontrol effect is remarkable.

Description

technical field [0001] The invention relates to the technical field of biocontrol bacteria, in particular to a strain of Staphylococcus aureus HN17496, a biocontrol agent and a preparation method and application thereof. Background technique [0002] Bipolarissorokiniana is a widely distributed pathogenic fungus in the world and is the main pathogen of wheat root rot. Pathogens can infect wheat roots, stem bases, leaves and ears, seriously affecting the yield and quality of wheat. At present, the control methods of Helminthium umbiliferus are mainly soaking seeds with chemicals or spraying chemical agents, all of which are harmful to the environment. There are few researches on the biocontrol of Helminthium umbiliferum, 2010 In 2008, a bacterium, Stenotrophomonas maltophilia C3 strain, was discovered. This strain can produce chitin-degrading enzymes and has a good control effect on Helminthosporium umbilis. However, this bacterium is an opportunistic pathogen, which is not...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30C12N1/145C12R2001/645A01P3/00
Inventor 耿月华高巍翟妮平张坤普张猛徐超郭雅双康国章
Owner HENAN AGRICULTURAL UNIVERSITY
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