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Preparation method and application of collagen peptide magnesium chelate

A collagen peptide and magnesium chelate technology, applied in the biological field, can solve the problems of osteoporosis, bone formation and bone resorption imbalance, and achieve the effect of improving antioxidant activity, avoiding side effects, and low operating process

Pending Publication Date: 2022-07-22
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the excessive accumulation of free radicals in the cells will also lead to the imbalance of bone formation and bone resorption in the organism, eventually leading to the occurrence of osteoporosis

Method used

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  • Preparation method and application of collagen peptide magnesium chelate
  • Preparation method and application of collagen peptide magnesium chelate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Step 1: Preparation of bovine collagen peptides

[0031] Take a certain amount of defatted and decalcified beef bones, add deionized water at a ratio of 1:4, adjust the pH of the solution to 8.0, add alkaline protease and papain at 1.0% and 0.75% of the mass of the beef bones, respectively, at 60 ℃ Enzymatic hydrolysis was carried out for 2 h, and then the enzyme was inactivated by boiling water bath for 15 min. Filtration dialysis was filtered through filter cotton, and then the ions introduced by adjusting the pH during the reaction were removed through a 200 Da dialysis bag, concentrated by a rotary evaporator, and rotary evaporated. The temperature is 60°C; then freeze-drying is performed, and the conditions for freeze-drying are cold hydrazine-60°C, and the drying chamber is 10°C, and then bovine collagen peptide P is prepared.

[0032] Step 2: Preparation of bovine collagen peptide magnesium chelate

[0033] The prepared bovine collagen peptide is dissolved at a ...

Embodiment 2

[0035] Step 1: Preparation of bovine collagen peptides

[0036] Take a certain amount of defatted and decalcified beef bones, add deionized water at a ratio of 1:4 to the liquid, adjust the pH of the solution to 8.0, add alkaline protease and papain at 0.75% and 0.75% of the mass of the beef bones, respectively, at 60 ℃ Enzymatic hydrolysis was carried out for 2 h, and then the enzyme was inactivated by boiling water bath for 15 min. Filtration dialysis was filtered through filter cotton, and then the ions introduced by adjusting the pH during the reaction were removed through a 200 Da dialysis bag, concentrated by a rotary evaporator, and rotary evaporated. The temperature is 60°C; then freeze-drying is performed, and the conditions for freeze-drying are cold hydrazine-60°C, and the drying chamber is 10°C, and then bovine collagen peptide P is prepared.

[0037] Step 2: Preparation of bovine collagen peptide magnesium chelate

[0038] The prepared bovine collagen peptide was...

Embodiment 3

[0040] Step 1: Preparation of bovine collagen peptides

[0041] Take a certain amount of defatted and decalcified beef bones, add deionized water at a ratio of 1:4 to the liquid, adjust the pH of the solution to 8.0, add alkaline protease and papain at 1.0% and 0.5% of the mass of the beef bones, respectively, at 60° C. Enzymatic hydrolysis was carried out for 2 h, and then the enzyme was inactivated by boiling water bath for 15 min. Filtration dialysis was filtered through filter cotton, and then the ions introduced by adjusting the pH during the reaction were removed through a 200 Da dialysis bag, concentrated by a rotary evaporator, and rotary evaporated. The temperature is 60°C; then freeze-drying is performed, and the conditions for freeze-drying are cold hydrazine-60°C, and the drying chamber is 10°C, and then bovine collagen peptide P is prepared.

[0042] Step 2: Preparation of bovine collagen peptide magnesium chelate

[0043]The prepared bovine collagen peptide was ...

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Abstract

The invention relates to a preparation method and application of a collagen peptide magnesium chelate. The preparation method comprises the following steps: step 1, preparing bovine bone collagen peptide; and step 2, preparing the bovine bone collagen peptide magnesium chelate. According to the method, cattle bones which are wide in source are used as raw materials, the cattle bone collagen peptide is prepared through double-enzyme composite enzymolysis, and the collagen peptide magnesium chelate is prepared through chelation of the cattle bone collagen peptide and magnesium ions. The collagen peptide magnesium chelate has significantly improved antioxidant activity and osteoblast promoting activity, can play a positive effect on prevention and treatment of osteoporosis, and provides a new thought for development and utilization of novel peptide magnesium chelate products.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method and application of a collagen peptide magnesium chelate. Background technique [0002] Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are free radicals that can play an important role in the human body, participating in intracellular signaling and host defense against invading pathogens. In the normal physiological state of the human body, the generation and elimination of free radicals are in a dynamic equilibrium state. When the body is affected by external factors or is in a pathological state, the dynamic balance of free radicals in the body may be broken. It can cause oxidative damage, which may lead to various diseases, such as heart disease, Alzheimer's disease, arteriosclerosis, diabetes and cancer. At the same time, the excessive accumulation of free radicals in cells can also lead to the imbalance of bone formation and bone resorpti...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61K33/14A61K33/06A61P19/10A61P39/06A61K8/65A61K8/19A61K8/23A61Q19/08C12P21/06C07K14/78C07K1/34C07K1/14C07K1/30C07K1/36
CPCA61K38/39A61K33/14A61K33/06A61P19/10A61P39/06A61K8/65A61K8/19A61K8/23A61Q19/08C12P21/06C07K14/78A61K2300/00
Inventor 黄雅钦张超
Owner BEIJING UNIV OF CHEM TECH
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