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Composite functional bacterial agent for in-situ remediation of contaminated soil by taking pseudomonas stutzeri as carrier and application of composite functional bacterial agent

A technology of Pseudomonas stutzeri and compound functional bacteria, which is applied in the field of bioremediation to maintain the diversity and persistence of soil microorganisms, shorten the treatment cycle, and improve the survival rate

Pending Publication Date: 2022-07-08
BEIJING VICTEX ENVIRONMENTAL PROTECTION TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a kind of composite functional bacterial agent and its application of Pseudomonas stutzeri as a carrier for in-situ restoration of contaminated soil, so as to improve the ability of the above-mentioned composite bacterial agent carrier to assist microorganisms to complete hydrocarbons in a long period of time and in a large range. Aggregation and degradation problems, thereby improving the degradation efficiency of petroleum hydrocarbons in soil

Method used

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  • Composite functional bacterial agent for in-situ remediation of contaminated soil by taking pseudomonas stutzeri as carrier and application of composite functional bacterial agent

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Effect test

Embodiment 1

[0046] The present embodiment provides a preparation method of a composite functional bacterial agent, and the method comprises the following steps:

[0047] S1. Preparation of fermentation medium

[0048] Add 40g of glucose, 15g of sodium nitrate, 6.5g of potassium dihydrogen phosphate, 6g of disodium hydrogen phosphate, 2g of peptone, and 340ml of biodiesel to the 30L pilot-scale fermenter, respectively, and add water to make the volume to 17L.

[0049] In the present embodiment, a degreasing filter is installed at the exhaust port of the fermenter, and after the conventional sterilization of the fermenter, the pH of the above-mentioned culture medium is adjusted to 7.0 by using sodium hydroxide with a mass concentration of 20%.

[0050] S2. Culture Pseudomonas stutzeri VTCQ-1 and Pseudomonas aeruginosa VTS-1

[0051] First, 425ml of Pseudomonas aeruginosa strain VTS-1 was inserted, and the culture conditions were: constant temperature of 37°C, stirring at 190rpm, and air v...

Embodiment 2

[0057] The present embodiment provides a preparation method of a composite functional bacterial agent, and the method comprises the following steps:

[0058] S1. Preparation of fermentation medium

[0059] To 30m 3 Add 40kg of glucose, 15kg of sodium nitrate, 6.5kg of potassium dihydrogen phosphate, 6kg of disodium hydrogen phosphate, 2kg of peptone, and 360L of biodiesel to the pilot-scale fermenter, and add water to make the volume to 18 m 3 .

[0060] In the present embodiment, a degreasing filter is installed at the exhaust port of the fermenter, and after the conventional sterilization of the fermenter, the pH of the above-mentioned culture medium is adjusted to 7.0 by using sodium hydroxide with a mass concentration of 20%.

[0061] S2. Culture Pseudomonas stutzeri VTCQ-1 and Pseudomonas aeruginosa VTS-1

[0062] First access 0.4m 3 Pseudomonas aeruginosa strain VTS-1, culture conditions: constant temperature 37°C, stirring 200rpm, air volume 0.3m 3 / min, tank press...

Embodiment 3

[0067] The present embodiment provides a method for remediating polluted soil in situ, comprising the following steps:

[0068] The microbial inoculum product obtained in Example 1 is added to the water according to the consumption of 0.5‰ and stirred, that is, the microbial inoculum using liquid;

[0069] The polluted soil retrieved from the underground of a gas station (the average petroleum hydrocarbon content of the three-dimensional sampling points within the pollution range is 1328 mg / m 3 ), put it into a glass column with a diameter of 0.25m and a height of 1.0m, and compacted it close to the original state of the soil. Use a metal tube with a porous wall to drill a hole from the middle and insert it into the center of the soil in the device, and inject 40L of the inoculant solution. Multi-site soil samples were regularly tested for bacterial density and petroleum hydrocarbon content.

[0070] After the inoculant was added to the soil, the two types of bacteria were t...

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Abstract

The invention discloses a composite functional bacterial agent for in-situ remediation of contaminated soil by using pseudomonas stutzeri as a carrier and application of the composite functional bacterial agent, the composite functional bacterial agent comprises pseudomonas stutzeri VTCQ-1 and pseudomonas aeruginosa VTS-1, the preservation number of the pseudomonas stutzeri VTCQ-1 is CGMCC24469, the preservation number of the pseudomonas aeruginosa VTS-1 is CGMCC2200, the preservation number of the pseudomonas stutzeri VTCQ-1 is CGMCC24469, and the preservation number of the pseudomonas aeruginosa VTS-1 is CGMCC2200. Wherein the pseudomonas stutzeri VTCQ-1 is used as a carrier of the pseudomonas aeruginosa VTS-1. According to the characteristics that the specific surface area of a bacterial colony of the pseudomonas stutzeri VTCQ-1 is large and a large and stable attachment place can be provided for other bacteria, the pseudomonas stutzeri VTCQ-1 and the pseudomonas aeruginosa VTS-1 are subjected to mixed culture, the survival rate of the pseudomonas aeruginosa VTS-1 can be increased, the prepared composite functional bacterial agent can improve the degradation efficiency of hydrocarbons in soil, and the degradation efficiency of the hydrocarbons in the soil is improved. The hydrocarbon polluted soil treatment period is greatly shortened, and the diversity and durability of soil microorganisms are maintained.

Description

technical field [0001] The present invention relates to the field of bioremediation of petroleum hydrocarbon-contaminated soil, in particular to a composite functional bacterial agent for in-situ remediation of contaminated soil using Pseudomonas stutzeri as a carrier and its application. Background technique [0002] At present, there are many methods in the field of in-situ remediation of hydrocarbon-contaminated soil, such as physical, chemical, and microbial methods. Among them, microbial in-situ remediation of hydrocarbon-contaminated soil is the most environmentally friendly, economical, free of secondary pollution, and more reliable in terms of sustainability and environmental friendliness. This method has attracted extensive attention and in-depth research in various environmental protection fields around the world. [0003] The key to microbial remediation methods is the selection of efficient hydrocarbon-degrading bacteria and the maintenance of bacterial flora in ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/385C12R1/38
CPCC12N1/20B09C1/10B09C2101/00Y02W10/10
Inventor 寇焕起金艳方寇秋莉
Owner BEIJING VICTEX ENVIRONMENTAL PROTECTION TECH DEV
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