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Monodisperse gelatin chitosan composite embolism microsphere with controllable degradation performance and elasticity and preparation method of monodisperse gelatin chitosan composite embolism microsphere

A monodisperse, gelatin shell technology, applied in the field of biomedicine, can solve the problems of poor sphericity, uncontrollable degradability and elasticity, poor biocompatibility, etc. small effect

Pending Publication Date: 2022-07-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of poor sphericity, poor monodispersity, poor biocompatibility, and uncontrollable degradability and elasticity of existing gelatin-chitosan composite microspheres, the present invention provides a monodisperse microsphere with adjustable degradation performance and elasticity. Gelatin-chitosan composite embolic microspheres and a preparation method thereof, in order to improve the monodispersity of the composite embolic microspheres while realizing continuous and non-toxic preparation of the composite embolic microspheres, reduce the possibility of misembolization, and realize gelatin-chitosan Controllable regulation of degradation time and elasticity of composite embolic microspheres

Method used

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  • Monodisperse gelatin chitosan composite embolism microsphere with controllable degradation performance and elasticity and preparation method of monodisperse gelatin chitosan composite embolism microsphere
  • Monodisperse gelatin chitosan composite embolism microsphere with controllable degradation performance and elasticity and preparation method of monodisperse gelatin chitosan composite embolism microsphere
  • Monodisperse gelatin chitosan composite embolism microsphere with controllable degradation performance and elasticity and preparation method of monodisperse gelatin chitosan composite embolism microsphere

Examples

Experimental program
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Effect test

Embodiment 1

[0055] In the present embodiment, the preparation of monodisperse gelatin-chitosan composite embolization microspheres is as follows:

[0056] (1) Preparation of inner phase, outer phase fluid and collection solution

[0057] Preparation of internal phase fluid: add gelatin and chitosan into acetic acid aqueous solution with a concentration of 0.32 mol / L, stir in a water bath at 40 ° C until completely dissolved, then cool to room temperature, put it in a vacuum drying box and vacuumize at room temperature to Remove the bubbles in the solution to obtain an internal phase fluid; in the internal phase fluid, the mass ratio of acetic acid aqueous solution, gelatin, and chitosan is 1:0.1:0.02;

[0058] Preparation of external phase fluid: dissolving oil-soluble surfactant Span80 in n-octanol to obtain an external phase fluid; in the external phase fluid, the mass ratio of n-octanol to Span80 is 1:0.04.

[0059] Preparation of collection solution: dissolving cross-linking agent Ge...

Embodiment 2

[0071] In the present embodiment, the preparation of monodisperse gelatin-chitosan composite embolization microspheres is as follows:

[0072] (1) Preparation of inner phase, outer phase fluid and collection solution

[0073] The same as the inner phase fluid, outer phase fluid and collection liquid in Example 1.

[0074] (2) Preparation of monodisperse gelatin-chitosan composite embolization microspheres

[0075] using a structure such as figure 2 The microfluidic device shown, the inner diameter of the outlet of the injection tube is 60 μm, and the inner diameter of the collection tube is 300 μm. The temperature of the constant humidity box was 40°C.

[0076] The inner phase fluid is input into the injection tube of the microfluidic device with a syringe pump, and the outer phase fluid is input into the collection tube of the microfluidic device with a syringe pump, and a monodispersed W / O emulsion is formed in the collection tube, using a syringe filled with collection ...

Embodiment 3

[0083] In the present embodiment, investigate the influence of the content of genipin in the collection solution on the preparation of gelatin-chitosan plug composite plug microspheres, and the steps are as follows:

[0084] (1) Preparation of inner phase, outer phase fluid and collection solution

[0085] The inner phase fluid and outer phase fluid are the same as in Example 1. The following four collection solutions with different genipin contents were prepared:

[0086] Different amounts of genipin were dissolved in the same amount of n-octanol to obtain collection solutions A, B, C, D; in collection solution A, the mass ratio of n-octanol and genipin dissolved was 1:0.0025; In solution B, the mass ratio of n-octanol and genipin solution is 1:0.005; in collection solution C, the mass ratio of n-octanol and genipin solution is 1:0.0075; in collection solution D, n-octanol and The mass ratio of genipin is 1:0.01.

[0087] (2) Preparation of gelatin-chitosan composite embol...

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Abstract

The invention provides a monodisperse gelatin chitosan composite embolism microsphere with adjustable degradation performance and elasticity, the composite embolism microsphere comprises crosslinked chitosan and gelatin, the crosslinked chitosan is used as a skeleton, the gelatin is distributed in a three-dimensional network structure of the skeleton, the composite embolism microsphere is spherical, and the particle size variation coefficient does not exceed 5%. The degradation performance, the elasticity and the swelling performance of the composite embolism microsphere are adjusted by adjusting the mass ratio of the cross-linked chitosan to the gelatin in the composite embolism microsphere and the cross-linking density of the cross-linked chitosan. The invention also provides a preparation method of the composite embolism microsphere based on a microfluidic technology. According to the preparation method disclosed by the invention, the monodispersity of the composite embolism microspheres can be improved while continuous and non-toxic preparation of the composite embolism microspheres is realized, the possibility of false embolism is reduced, and controllable adjustment of degradation time and elasticity of the gelatin chitosan composite embolism microspheres is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, relates to embolization microspheres and a preparation process thereof, and more particularly relates to monodisperse gelatin-chitosan composite embolization microspheres with adjustable degradation performance and elasticity and a preparation method thereof. Background technique [0002] In the middle and late stages of solid tumors, surgery is often unresectable. Embolization therapy has become an alternative palliative treatment method. Embolization therapy refers to minimally invasive surgery to inject embolic materials into the blood vessels near the tumor to block the blood supply to the tumor. . Compared with ordinary chemotherapy or radiotherapy, embolization can reduce side effects, improve patient compliance and treatment efficiency. [0003] Solid embolization materials that can be used for embolization therapy include PVA, spring collar, etc., but their applications are limited d...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/08A61L24/00B01J13/02
CPCA61L24/001A61L24/043B01J13/02A61L2400/06C08L89/00C08L5/08
Inventor 褚良银杨仕豪巨晓洁邓传富汪伟谢锐刘壮潘大伟夏有强
Owner SICHUAN UNIV
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