Automatic RNA (Ribonucleic Acid) preparation device and preparation method

Abstract

The invention discloses an automatic RNA preparation device and method, the automatic RNA preparation device comprises a centrifugal device, a solution preparation device, a suction device and a control device, the centrifugal device forms a centrifugal cavity, the centrifugal cavity comprises a feeding chamber and a discharging chamber which are communicated with each other, the solution preparation device comprises a movably arranged mounting seat, the mounting seat is provided with a plurality of containing cavities, and the containing cavities are communicated with the feeding chamber and the discharging chamber. Each containing cavity is provided with a liquid outlet, so that the liquid outlets sequentially pass through and are communicated with the feeding cavities in the moving stroke of the mounting seat; the suction device comprises a barrel and a suction mechanism, the barrel is connected with the liquid storage cavity, the discharging end of the barrel is connected with the pipette tip, so that materials in a discharging cavity are sucked into the liquid storage cavity through the pipette tip when the suction mechanism sucks, and the control device is electrically connected with the suction device and the solution preparation device. The control device is used for controlling the centrifugal device, the suction device and the solution preparation device to move. The automatic RNA preparation device provided by the invention can realize automation and intelligentization of preparation and extraction of RNA.

Description

technical field [0001] The invention relates to the field of RNA preparation and extraction, in particular to an RNA automatic preparation device and preparation method. Background technique [0002] RNA extraction technology is widely used in key biomedical experiments such as PCR amplification. At present, the existing RNA automatic preparation devices are generally based on Trizol reagent method to extract RNA. The process of RNA extraction based on the Trizol reagent method involves several key steps such as adding Trizol reagent and chloroform, mixing and stirring, centrifuging the mixture, and suctioning the supernatant. These operation steps are currently mostly realized by manual operation, and it is easy to repeat the operation many times. Reduced operating accuracy and increased fatigue are not conducive to mass production. The current RNA extraction device relies on the human eye to identify the stratified interface of the mixed liquid after centrifugation, and t...

AI Technical Summary

Problems solved by technology

The process of RNA extraction based on the Trizol reagent method involves several key steps such as adding Trizol reagent and chloroform, mixing and stirring, centrifuging the mixed solution, and aspiration of the supernatant. These operating steps are currently mostly performed manually, and it is easy to repeat the operation many times. Reduced operating accuracy and increased fatigue, which is not conducive to mass production

The current RNA extraction device relies on human eyes to identify the layered interface of the centrifuged mixed solution, and then uses a pipette gun to draw the supernatant, which is inefficient in operation accuracy and efficiency

RNA can combine with protein and DNA. There is a small amount of protein pollution and DNA pollution in the external environment such as the air. The current RNA extraction device lacks a sealed environment, it is difficult to extract high-purity RNA, and there is no automatic dosage function. Reagents and The amount of cell fluid extraction basically depends on the volume range of the manual operation of the pipette

[0003] In the current RNA extraction device, the addition and extraction of reagents and cell fluids basically rely on manual and human eye recognition, relying on human eyes to recognize the layered interface of the mixed solution after centrifugation, which is inefficient and poor in accuracy

Although the fully sealed operation of the RNA extraction process has been realized in the prior art, the degree of automation is low, and manual operation is still required for the extraction and addition of reagents; the supernatant layer will appear in the mixed solution after centrifugation. The current technology Human eyes are used to identify the layered interface, and then the supernatant is sucked and discarded with a pipette gun, which is prone to operational errors and increases the workload of the operator; RNA extraction based on the Trizol reagent method has many steps and a long waiting time for each step , unable to achieve full automation and unattended

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