Preparation method of lactobacillus acidophilus powder capable of increasing culturable cell content
A technology of Lactobacillus acidophilus and cell culture, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effect of reducing the production of bacterial cells, increasing the number of bacterial cells, and simple operation
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Embodiment 1
[0043] The present embodiment provides a preparation method of Lactobacillus acidophilus bacteria powder that increases the content of culturable cells, and the preparation method comprises the following steps:
[0044] (1) Take out the glycerol tube of Lactobacillus acidophilus LA85 from the -80°C refrigerator, streak it on MRS agar medium by plate, and culture it anaerobically at 37°C for 72 h to obtain a single colony, which is the streak passage 1 Then, pick the largest colony on the plate and culture it again on MRS agar medium according to the above conditions to obtain a single colony. Record the colony size (unit: mm) with the largest diameter and the top 10 each time.
[0045] Table 1
[0046]
[0047] (2) The colony with a diameter of 4.2 mm obtained in step (1) (passage 15 times, colony 6) was inoculated into MRS liquid medium for activation and culture at 37 °C for 18 h, and then transferred with 2% of the inoculum. It was cultured in a 5 L fermenter containin...
Embodiment 2
[0050] This embodiment provides a method for preparing Lactobacillus acidophilus bacteria powder that increases the content of culturable cells. The only difference from Embodiment 1 is that in step (2), the diameter of the colony obtained in step (1) is 3.2 mm. Colonies (passage 8, colony 2) were activated for inoculation.
Embodiment 3
[0052] This embodiment provides a method for preparing Lactobacillus acidophilus bacteria powder that increases the content of culturable cells. The only difference from Embodiment 1 is that in step (2), the diameter of the colony obtained in step (1) is 3.7 mm. Colonies (passage 13, colony 5) were activated for inoculation.
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