Human venous thrombosis risk gene polymorphism detection kit, process and application

Abstract

The invention belongs to the technical field of polymorphism detection, and particularly relates to a human venous thrombosis risk gene polymorphism detection kit and process and application. PAI-1 4G / 5G and THBDc.-151Ggt are detected at the same time; t, and PROC 565Cgt; the invention discloses a kit for detecting the polymorphism of genes such as T (rs146922325) and PROC c.574576del, the kit comprises a reagent A, a reagent B, a positive quality control A, a positive quality control product B and a negative control, and the reagent A comprises a PAI-1 4G / 5G wild type and THBD c.-151Ggt; a specific primer, a common probe and a paired primer of the T wild type, PROC 565Cgt; t upstream and downstream primers, and PROC 565Cgt; according to the kit, a PCR-fluorescent probe method is adopted, a completely closed system is adopted for amplification reaction and detection, false positive caused by aerosol pollution is avoided, and the kit is simple in operation method, short in detection period, low in detection cost and good in sensitivity and specificity.

Description

technical field [0001] The invention relates to the technical field of polymorphism detection, in particular to a human venous thrombosis risk gene polymorphism detection kit, process and application. Background technique [0002] Venous thromboembolism is a multifactorial disease, and genetic factors play a very important role in the pathogenesis of VTE. Some studies have shown that the role of different genetic factors in the pathogenesis of VTE may account for more than 60%, and more than 50% have no incentives. Different types of genetic predisposition to thrombosis can be detected in VTE patients. [0003] PAI-1 protein is a major inhibitor of tissue plasminogen activator in plasma. There is an association between mutations at the PAI-14G / 5G (rs1799762) locus and an increased risk of VTE in individuals. The 4G allele of the PAI-1 gene can increase the plasma PAI-1 concentration by up-regulating the expression of the PAI-1 gene, and the patient is in a hypercoagulable ...

AI Technical Summary

Problems solved by technology

Among them, although the sequencing method and chip method can detect multiple genes and multiple loci at one time, the operation is cumbersome, the detection cycle is long, and the cost is relatively high, which is not conducive to clinical promotion.

The restriction fragment length peptide method requires gel electrophoresis of the amplified product, which is not only cumbersome to operate, but also non-blocking management operations are likely to cause contamination and cause false positives

Although the high-resolution melting curve method is easy to operate, it has low specificity and requires high equipment

ARMS method and Taqman fluorescent probe method are easy to operate and have high sensitivity, but they have poor specificity for single-base mutations, especially for single-base mutations in genes with high GC content, which will lead to non-specific amplification and may produce false positives. positive result

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