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Preparation method of DNA hydrogel loaded with IL-33 as well as product and application of DNA hydrogel loaded with IL-33

A technology of IL-33 and hydrogel, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, pharmaceutical formulas, etc., to improve the therapeutic effect, promote wound healing, and reduce the cycle of drug application

Active Publication Date: 2022-06-28
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of the present invention is to provide a preparation method of IL-33-loaded DNA hydrogel and its products and applications, and the process adopted for its preparation is simple , its products have slow-release, anti-oxidation and self-degradation capabilities when used in wound healing. This product can also be especially targeted at the treatment of difficult wound healing such as diabetes

Method used

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  • Preparation method of DNA hydrogel loaded with IL-33 as well as product and application of DNA hydrogel loaded with IL-33
  • Preparation method of DNA hydrogel loaded with IL-33 as well as product and application of DNA hydrogel loaded with IL-33
  • Preparation method of DNA hydrogel loaded with IL-33 as well as product and application of DNA hydrogel loaded with IL-33

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] see figure 1 . The preparation method of a DNA hydrogel loaded with IL-33 described in this example includes the following steps:

[0037] S1) Preparation: including recombinant mouse IL-33 and 5 kinds of single-stranded DNA as shown in SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4 and SEQ ID No.5, The concentration of the recombinant mouse IL-33 is 0.5ng / μL;

[0038] S2) Dissolution: each of the 5 single-stranded DNAs described in S1) was dissolved in 1×PBS buffer, the final concentration of the obtained lysing solution was 1 mmol / L, and 1 mmol / L of MgCl was added to the 1×PBS buffer. 2 , the pH of the 1xPBS buffer is 7.2;

[0039] S3) Construction of the monomer: the solubilization obtained in S2) by dissolving the single-stranded DNA shown in SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3 in the 1xPBS buffer described in S2), respectively After the liquid is mixed, a mixture with a concentration of 250 μmol / L is first subjected to constant temperature treatment...

Embodiment 2

[0043] see figure 1 . The preparation method of a DNA hydrogel loaded with IL-33 described in this example includes the following steps:

[0044] S1) Preparation: including recombinant mouse IL-33 and 5 kinds of single-stranded DNA as shown in SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4 and SEQ ID No.5, The concentration of the recombinant mouse IL-33 is 1ng / μL;

[0045] S2) dissolving: the 5 kinds of single-stranded DNAs described in S1) are respectively dissolved in 1×PBS buffer solution, the final concentration of the obtained lysing solution is 2mmol / L, and 2mmol / L MgCl is added to this 1×PBS buffer solution 2 , the pH of the 1×PBS buffer is 8.0;

[0046] S3) Construction of the monomer: the solubilization obtained in S2) by dissolving the single-stranded DNA shown in SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3 in the 1xPBS buffer described in S2), respectively After the liquid is mixed, the mixture with a concentration of 300 μmol / L is first subjected to consta...

Embodiment 3

[0050] A non-denaturing polyacrylamide gel electrophoresis method described in this example is used to determine whether the Y monomer and L monomer described in S3) in Example 1 are successfully constructed, and the steps include:

[0051] a) Prepare PAGE gel electrophoresis buffer;

[0052] b) Preparation of separating gel and stacking gel required for non-denaturing polyacrylamide gel electrophoresis;

[0053] c) performing native polyacrylamide gel electrophoresis;

[0054] d) Analyze the electrophoresis results, and judge whether the Y monomer and the L monomer are successfully constructed according to the general standard analysis method.

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Abstract

The invention discloses a preparation method of IL-33-loaded DNA hydrogel as well as a product and application of the IL-33-loaded DNA hydrogel, and belongs to the field of pharmaceutical preparations. The invention aims to provide the preparation method of the IL-33-loaded DNA hydrogel with a simple preparation process, and the product has slow release, anti-oxidation and self-degradation capabilities when being applied to wound healing. According to the technical scheme, the preparation method of the DNA hydrogel loaded with the interleukin IL-33 comprises the following steps: S1) preparing materials; s2) dissolving; s3) constructing a Y monomer and an L monomer; and S4) incubating and mixing.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a preparation method of a DNA hydrogel loaded with IL-33 and its product and application. Background technique [0002] After the skin and its deep tissues are injured, the detachment or defect will lead to the formation of wounds. The recovery and healing process of wounds is generally called wound healing or wound healing. A complex combination that exhibits a synergistic realization of various processes. Wound healing is the process of wound regeneration and repair. The basic process of wound healing includes acute inflammation, cell proliferation, scar formation, and regeneration of epidermis and other tissues. However, some wounds will continue to be in an acute inflammatory phase and aggravate the inflammatory process due to their own particularities, such as decubitus / pressure ulcers and poor healing of burns / scalds; or as complications of diseases s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/20A61K9/06A61K47/26A61P17/02A61P3/10A61P17/18
CPCA61K38/20A61K9/06A61K47/26A61P17/02A61P3/10A61P17/18
Inventor 郑晓峰王成世李威王政昊
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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