Culture medium for tissue culture of Tin-Gong type chenopodium camellioides

A technology of tissue culture and culture medium, applied in the field of plant biology, can solve the problems of shortening the breeding cycle, low survival rate, slow reproduction speed, etc., and achieve the effect of increasing the reproduction speed, high cultivation efficiency and low cost

Pending Publication Date: 2022-06-28
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently on the market, most of the planting methods for Xilin Gol type camel's quinoa are seed planting and planting, the propagation speed is slow and the survival rate is generally low, and tissue culture technology can obtain a large number of Xilingol type camel's seedlings in a short period of time, shortening the breeding cycle, It is an effective way for the Xilin Gol type camel's hair to meet the market demand. The invention provides a culture medium for the Xilin Gol type of camel's hair tissue culture, which has important guiding significance for the rapid propagation and large-scale promotion of the Xilin Gol type of camel's hair

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Select the 4th leaf under the growth point of the Xilin Gol-type Camelina branch as an explant, sterilize with 75% alcohol for 30 seconds, sterilize with mercuric chloride solution for 4 minutes, and finally wash it with sterile water for 5 times, each time for 30 seconds;

[0020] The sterilized explants were inoculated into the induction medium to induce clustered buds, and the formula of the induction medium was: first-generation improved MS+0.18mg / L 6-BA+0.03mg / L NAA;

[0021] The formulation of the first-generation improved MS is as follows: the content of calcium (Ca) and magnesium (Mg) in the original MS medium is reduced by 1.5 mg / L.

[0022] Measure the induction rate of its cluster buds, see Table 1 for details.

Embodiment 2

[0024] Select the 4th leaf under the growth point of the Xilin Gol-type Camelina branch as an explant, sterilize with 75% alcohol for 30 seconds, sterilize with mercuric chloride solution for 4 minutes, and finally wash it with sterile water for 5 times, each time for 30 seconds;

[0025] Inoculate the sterilized explants into the induction medium to induce clustered buds. The formula of the induction medium is: the first generation of improved MS+0.23mg / L 6-BA+0.08mg / L NAA; the first generation of improved The formula of MS is: reduce the content of calcium (Ca) and magnesium (Mg) in the original MS medium by 1.5mg / L;

[0026] Measure the induction rate of its cluster buds, see Table 1 for details.

Embodiment 3

[0028] Select the 4th leaf under the growth point of the Xilin Gol-type Camelina branch as an explant, sterilize with 75% alcohol for 30 seconds, sterilize with mercuric chloride solution for 4 minutes, and finally wash it with sterile water for 5 times, each time for 30 seconds;

[0029] The sterilized explants were inoculated into the induction medium to induce clustered buds, and the formula of the induction medium was: first-generation improved MS+0.2mg / L 6-BA+0.05mg / L NAA;

[0030] The formulation of the first-generation improved MS is as follows: the content of calcium (Ca) and magnesium (Mg) in the original MS medium is reduced by 1.5 mg / L.

[0031] Measure the induction rate of its cluster buds, see Table 1 for details.

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PUM

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Abstract

The invention provides a culture medium, an induction culture medium and a subculture medium for tissue culture of Yin Guille type Chenopodium hupehensis. The formula of the induction culture medium is as follows: primary improved MS, 0.18-0.23 mg / L of 6-BA and 0.03-0.08 mg / L of NAA; according to the formula of the primary improved MS, the content of calcium (Ca) and the content of magnesium (Mg) in an original MS culture medium are reduced by 1.5 mg / L; the formula of the subculture medium is as follows: the subculture improved MS, the 6-BA with the concentration of 0.28 to 0.33 mg / L and the NAA with the concentration of 0.03 to 0.08 mg / L; the formula of the subculture improved MS is as follows: major elements in an original MS culture medium are reduced by 1.5 mg / L, and other components are not changed; according to the invention, nutritional ingredients and proportions of the culture medium required in each period are provided, and theoretical and practical foundations are laid for large-scale popularization and application of the Tin-Godler type chenopodium camellioides.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a culture medium for tissue culture of Xilin Gol type camel's quinoa. Background technique [0002] Grassland is the main body of Inner Mongolia's ecology. Xerophytic pasture is an important material basis for grassland ecological conservation and sustainable development. However, in the current production and ecological utilization, insufficient attention is paid to the planting resources of xerophytic pasture, and there is a serious shortage of plants, which cannot meet the needs of the market. need. [0003] Xilin Gol type Camelina is a perennial xerotic semi-shrub of Chenopodiaceae, which is suitable for ecological restoration of degraded grassland and sandy land in arid and semi-arid areas, construction of shrub protection belts on grass pastures, and construction of farmland biological hedges. It not only has low planting costs, but also The cost of managemen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002
Inventor 王召明马怀林贾振宇金净刘亚玲张跃华闫士元屈璐璐阿拉塔白杨王晓龙
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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