Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of lactobacillus casei LC89 and microbial preparation containing lactobacillus casei LC89 in preparation of product for relieving functional constipation

A technology of Lactobacillus casei and functional constipation, applied in the field of microorganisms, can solve unknown problems, achieve excellent results, increase the number of viable bacteria, and promote growth

Pending Publication Date: 2022-06-24
JIANGSU WECARE BIOTECHNOLOGY CO LTD
View PDF9 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although Lactobacillus casei and other bacterial strains in the prior art can regulate intestinal health and have the potential to treat constipation, it is still unknown whether they are effective for functional defecation disorders, a special subtype of constipation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of lactobacillus casei LC89 and microbial preparation containing lactobacillus casei LC89 in preparation of product for relieving functional constipation
  • Application of lactobacillus casei LC89 and microbial preparation containing lactobacillus casei LC89 in preparation of product for relieving functional constipation
  • Application of lactobacillus casei LC89 and microbial preparation containing lactobacillus casei LC89 in preparation of product for relieving functional constipation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Culture of Lactobacillus casei LC89

[0069] Lactobacillus casei LC89 was inoculated into a modified MRS liquid medium (ie, prebiotic-added MRS liquid medium) with a 2% inoculum, and after culturing at 37°C for 16 h, it was transferred to a fresh modified MRS liquid medium for 37 hours. After culturing for 16 hours at ℃, the cells were collected by centrifugation at 10,000 g for 5 minutes, washed with 0.9% saline, and centrifuged again at 10,000 g for 5 minutes to obtain a Lactobacillus casei LC89 culture, which was resuspended in a mixed solution of 40% glycerol and modified MRS liquid medium. , to obtain Lactobacillus casei LC89 bacterial suspension, frozen at -80 ℃ for later use.

[0070] The formula of the improved MRS liquid culture medium is: in parts by mass, peptone 1%, beef extract 1%, glucose 2%, prebiotics 1.7%, yeast extract 0.5%, diammonium hydrogen citrate 0.2%, K 2 PO 4 ·3H 2 O0.2%, MgSO 4 ·7H 2 O0.058%, MnSO 4 0.019%, L-cysteine ​​0.1%, Tween-80 0...

Embodiment 2

[0075] Gastric acid resistance test of Lactobacillus casei LC89

[0076] Preparation of artificial gastric juice: in terms of mass fraction, artificial gastric juice contains 0.20% NaCl and 0.30% pepsin, adjust pH to 2.0, 2.5 and 3.0 with HCl, filter sterilization for later use;

[0077] The preparation method of Lactobacillus casei LC89 bacterial suspension refers to Example 1, wherein the prebiotic in the culture medium is a combination of inulin and galactooligosaccharide, and the mass ratio is 10:7.

[0078] Gastric acid resistance test: take 1.0 mL of Lactobacillus casei LC89 bacterial suspension (concentration of 1 × 10 9 CFU / mL, using the method in the national standard "GB4789.35-2016 National Food Safety Standard for Food Microbiology Detection of Lactic Acid Bacteria" to measure the concentration of bacterial liquid), mixed with 9.0mL of artificial gastric juice with pH of 2.0, 2.5 and 3.0, respectively, at 37 Anaerobic static culture at ℃, samples were taken at the...

Embodiment 3

[0081] Establishment of a functional constipation rat model

[0082] 48 clean-grade SD rats, weighing 230 g ± 20 g, were provided by the Shanghai Laboratory Animal Research Center and have been approved by the Animal Ethics Committee of the Shanghai Laboratory Animal Research Center (ethics number: 2021082003).

[0083] Grouping and administration: Rats were randomly divided into normal group (8 rats) and model group (40 rats). The model group was randomly divided into 5 dose groups (8 rats in each group). 28mg / kg. Compound diphenoxylate tablets (purchased from Changzhou Kangpu Pharmaceutical Co., Ltd.) were ground into fine powder with a mortar, and then prepared into a suspension with distilled water according to the concentration required by the 5 dosage groups. After 12 hours of fasting, the rats were given 10 mL / kg of intragastric gavage once a day for 6 weeks.

[0084] Evaluation criteria for functional constipation rats: the clinical manifestations and defecation rec...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of lactobacillus casei LC89 and a microbial preparation containing the lactobacillus casei LC89 in preparation of a product for relieving functional constipation. The lactobacillus casei LC89 is creatively found to have an excellent effect in the aspect of treating functional constipation which is a special constipation subtype, the treatment effect of the lactobacillus casei LC89 is superior to that of bifidobacterium lactis BLa80 and other strains which are disclosed in the prior art and have the potential of improving constipation, and the lactobacillus casei LC89 has important application value in preparation of products for relieving functional constipation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to the application of Lactobacillus casei LC89 and a microbial preparation containing the same in the preparation of products for relieving functional constipation. Background technique [0002] Although the frequency of bowel movements varies from person to person, most people have regular bowel movements without significant strain or discomfort. However, some people have functional defecation disorder, namely functional constipation (FC), also known as chronic idiopathic constipation (CIC), which is a common gastrointestinal disease. Manifested by persistent difficulty in defecation, decreased frequency of defecation, or a feeling of incomplete defecation. FC is usually not caused by organic diseases or drug factors, but is usually multifactorial, including living habits, mental and psychological factors, environmental conditions, and eating habits. [0003] According to th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23L2/39A23C9/123C12N1/20A61K35/747A61P1/10C12R1/245C12R1/25C12R1/225C12R1/46C12R1/01
CPCA23L33/135A23L2/39A23C9/1234C12N1/20A61K35/747A61P1/10A23V2002/00A23V2400/125A23V2250/628A23V2250/61A23V2250/5072A23V2250/042A23V2250/264
Inventor 方曙光董瑶盖忠辉陈珂可朱建国程文
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com