Preparation and staining methods of pathological tissue section staining kit

Abstract

The invention relates to the technical field of pathological tissue section staining, and discloses a preparation method of a pathological tissue section staining kit, and the staining kit comprises a filtered stabilizing solution, a hematoxylin staining solution, a differentiation solution, a blue returning solution and an eosin staining solution; the stabilizing solution comprises 2-20 parts of organic acid, 0-300 parts of polyhydric alcohol and 700-1000 parts of water, and the pH value of the stabilizing solution is adjusted to 2.5-3.5 through a sodium hydroxide solution; the hematoxylin dye liquor is prepared from 2 to 10 parts of hematoxylin, 150 to 300 parts of polyhydric alcohol, 0.2 to 1.0 part of oxidizing agent, 30 to 100 parts of mordant, 1 to 20 parts of organic acid and 700 to 850 parts of water. According to the preparation method of the pathological tissue section staining kit and the hematoxylin staining solution, by adjusting the proportion of mordant in the components, no metal film or precipitate is generated in the using process, the workload of technicians is greatly reduced, the cell nucleus staining efficiency is improved, the kit can be used for a long time, meanwhile, cell nucleus chromatin staining is meticulous, and the staining effect is good. The cell nucleus dyeing effect is improved.

Description

technical field [0001] The invention relates to the technical field of pathological tissue section dyeing, in particular to a preparation and dyeing method of a pathological tissue section dyeing kit. Background technique [0002] Hematoxylin-eosinstaining, referred to as HE staining, is one of the commonly used staining methods in paraffin section technology. The hematoxylin staining solution is alkaline, which mainly makes the chromatin in the nucleus and the nucleic acid in the cytoplasm violet blue; eosin is an acidic dye, which mainly makes the components in the cytoplasm and the extracellular matrix red. HE staining is the most basic and widely used technique in teaching and research of histology, embryology and pathology. The operation steps in the hematoxylin-eosin staining method mainly include sectioning - baking - dewaxing - rehydration - stabilizing solution - hematoxylin staining - washing - differentiation - washing - back to blue - washing - eosin staining - ...

AI Technical Summary

Problems solved by technology

[0005] 1. The surface of the hematoxylin staining solution is easy to produce a metal film, or there is dye precipitation, which will easily lead to stains on the surface of the slide after hematoxylin staining, which is difficult to remove, resulting in uneven staining and unreadable slides;

[0006] 2. For the metal film produced by hematoxylin staining solution, it needs to be removed by filtration, which increases the workload of technicians and wastes time and reagents;

[0007] 3. Nucleocytoplasmic co-staining is prone to occur, and the staining effect is not good;

[0008] 4. Poor dyeing stability, easy to fade, can not meet the needs of long-term storage;

[0009] 5. In different seasons and temperatures, there are different requirements for dyeing time. It is necessary to explore the appropriate dyeing time before starting clinical application, which seriously reduces the use effect

Although the existing eosin dye formula is simple to operate, the dyeing time is long and the validity period of the dye solution is short, so it is often necessary to add glacial acetic acid to cooperate with it. If too much is added, the staining color of the slices will be incorrect and easy to fade, and the staining effect cannot be preserved for a long time

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