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Porcine reproductive and respiratory syndrome virus ultrafiltration purification system

A technology for respiratory syndrome and pig breeding, applied in the field of virus propagation, can solve problems such as side effects, achieve the effects of reducing blockage, reducing stress response, and good anti-pollution performance

Pending Publication Date: 2022-06-14
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main technical means for preventing and treating the disease is still the PRRS vaccine. The inactivated porcine reproductive and respiratory syndrome vaccine produced by conventional technology has certain side effects in clinical application, which are mainly related to the extraantigenic components contained in the virus antigen ( invalid protein)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Modified Montmorillonite

[0029] Stir and disperse 25g of montmorillonite in 500ml, 0.9wt% of physiological saline, heat to boiling, cool to room temperature, filter and wash, then stir and disperse in 600ml of deionized water, add dropwise 10ml, 5wt% of The tetramethylammonium chloride solution was reacted at 80-90° C. for 4 hours. After the reaction was completed, the modified montmorillonite was obtained by suction filtration, washing, drying and grinding.

[0030] Preparation of composite modified particles

[0031] S1: Stir and disperse 20g of modified montmorillonite in 150mL of acetone solution, add 5g of acrylic acid monomer, stir and mix uniformly with magnetic stirring, add 0.5g of cetyltrimethylamine bromide and 0.2g of benzamide peroxide , after stirring and mixing, the oil bath was heated to 110 ℃, the reaction was kept for 100 min, and then the acetone was removed in vacuum at room temperature to obtain material 1;

[0032] S2: stirring a...

Embodiment 2

[0037] The preparation of modified montmorillonite is the same as that of Example 1.

[0038] Preparation of composite modified particles

[0039] S1: Stir and disperse 20 g of modified montmorillonite in 150 mL of acetone solution, add 8 g of acrylic acid monomer, stir and mix uniformly with magnetic stirring, add 0.8 g of cetyltrimethylamine bromide and 0.2 g of benzamide peroxide , after stirring and mixing, the oil bath was heated to 120 ℃, the reaction was kept for 90 min, and then the acetone was removed in vacuum at room temperature to obtain material 1;

[0040] S2: stirring and dispersing 11 g of nanocellulose crystals in 100 mL of toluene, ultrasonically dispersing to obtain a suspension, placing the mixture in a ball mill, adding the suspension, ball milling at a speed of 300 rpm for 45 min, taking out, and recovering toluene under reduced pressure to obtain After the solid is dried, it is heated to 75°C by far-infrared and stirred during heating, and 20 mL of 1wt%...

Embodiment 3

[0045] The preparation of modified montmorillonite is the same as that of Example 1.

[0046] Preparation of composite modified particles

[0047] S1: Stir and disperse 20g of modified montmorillonite in 150mL of acetone solution, add 10g of acrylic monomers, stir and mix with magnetic force, add 0.6g of cetyltrimethylamine bromide and 0.1g of benzamide peroxide , after stirring and mixing, the oil bath was heated to 100 ℃, and the reaction was kept for 120 min, and then the acetone was removed in vacuum at room temperature to obtain material 1;

[0048] S2: stirring and dispersing 10 g of nanocellulose crystals in 100 mL of toluene, ultrasonically dispersing to obtain a suspension, placing the mixture in a ball mill, adding the suspension, ball milling at a speed of 250 rpm for 40 min, taking out, and recovering toluene under reduced pressure to obtain After the solid is dried, it is heated to 70°C by far-infrared heating and stirred during heating, and 20 mL of 1wt% chitosa...

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PUM

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Abstract

The invention relates to the technical field of virus reproduction, in particular to a porcine reproductive and respiratory syndrome virus ultrafiltration purification system which comprises a first concentration tank, a second concentration tank and a third concentration tank which are sequentially connected through pipelines. A first ultrafiltration membrane bag is arranged between the first concentration tank and the second concentration tank, a second ultrafiltration membrane bag is arranged between the second concentration tank and the third concentration tank, a first ultrafiltration membrane and a second ultrafiltration membrane are installed in the first ultrafiltration membrane bag and the second ultrafiltration membrane bag respectively, the aperture of the first ultrafiltration membrane is larger than the molecular weight of target viruses, and the aperture of the second ultrafiltration membrane is larger than the molecular weight of the target viruses. The aperture of the second ultrafiltration membrane is smaller than the molecular weight of a target virus. According to the porcine reproductive and respiratory syndrome virus ultrafiltration purification system disclosed by the invention, by arranging a three-stage filtration purification system, invalid proteins in vaccines can be removed by 95% or above, the antigen purity can be effectively improved, and the stress reaction of animals to the vaccines can be reduced.

Description

technical field [0001] The invention relates to the technical field of virus reproduction, in particular to an ultrafiltration and purification system for porcine reproductive and respiratory syndrome virus. Background technique [0002] Porcine reproductive and respiratory syndrome virus (PRRS) is a highly contagious infectious disease, and outbreaks of the disease are characterized by abortion (10%-50%), sow death (5%-10%), and commercial pigs. More than 50% of piglets die before weaning, and respiratory diseases are the characteristics. In recent years, the disease has shown an obvious high incidence trend in China, which has caused heavy losses to the pig industry and has become an important infection that seriously threatens the development of China's pig industry. one of the diseases. [0003] The pathogen of PRRS is porcine reproductive and respiratory syndrome virus (PRRSV), which is an RNA virus with a high mutation rate found so far. PRRSV can be transmitted both ...

Claims

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Application Information

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IPC IPC(8): B01D61/14B01D61/18B01D67/00B01D69/02B01D71/68C12M1/12C12N7/02C12R1/93
CPCB01D61/18B01D61/145B01D67/0079B01D71/68B01D69/02C12M47/02C12N7/00B01D2325/24B01D2325/36C12N2770/10051
Inventor 李阳沈建军彭俊平胡永明娄坤鹏张秀文陈雪屈建王悦芸
Owner 浙江美保龙生物技术有限公司
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