Multilayer alignment fusion method and application thereof

Abstract

The invention relates to a multilayer alignment fusion method and application thereof, and the scheme comprises the following steps: carrying out initial positioning on a slide to determine a cell range of a sample on the slide; dividing the slide into n rows and n columns of view images according to the view of the camera; setting a focusing point for the slide information according to the size of the current cell range; controlling the Z-axis movement of the camera to find a relatively clear focusing point as a clear point; performing fine focusing on each clear point to find the clearest point as a target point; fitting all target points into a smooth focusing clear surface; scanning the view image layer by layer according to the focusing clear surface; by taking the focusing clear surface as a reference, carrying out alignment correction on residual layers and fusing similar parts; and after all the rows and all the layers are scanned, splicing to form a panoramic high-definition image. The method has the advantages of high scanning definition and wide application range.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a multi-layer alignment fusion method and its application. Background technique [0002] In order to digitize the real slides and make it more convenient for doctors to read the slides, the slide scanning imaging method is adopted. However, at present, doctors often have cell stacking when making slides, and the information on the slide is thickness, rather than a flat surface. [0003] like figure 1 As shown, slide scanning is to place a whole physical slide in a slide scanner, and use an algorithm to divide the slide into several small field of view images according to the camera's field of view, find the surface where a clear focus point is located, and then according to the camera's field of view The zigzag scanning method scans line by line, and finally stitches and fuses it into a panoramic image. However, this method has the problems of slow response speed and insuf...

AI Technical Summary

Problems solved by technology

However, this method has the problems of slow response speed and insufficient precision, so the scanning time of the whole slide will be very slow, and the daily scanning volume is very low, which cannot meet the daily scanning volume of large hospitals.

And the existing technology does not take into account that the position of each layer of image is not the same due to hardware reasons when merging. For example, the existing three-layer scanning method uses the method of directly dividing the whole slice into N layers, and then scanning layer by layer. , does not take into account the error generated when each layer of the hardware scans and switches, the scanned images are not aligned, and the final fused image will be lost or misplaced during fusion

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