Nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen in acid response as well as preparation method and application of nano-drug

A continuous release, singlet oxygen technology, applied in the field of biomedical materials and nanomaterials, can solve the problems of limited glucose content, easy inactivation of enzyme activity, limited loading rate, etc., and achieve the effect of effectively inhibiting the growth of tumor cells

Pending Publication Date: 2022-05-27
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the loading rate of GOX in nanosystems is limited, its enzymatic activity is easily inactivated in complex biological environments, and the glucose content in tumor tissues is also extremely limited, so it is not conducive to the efficient production of H in tumor tissues. 2 o 2 substrate

Method used

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  • Nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen in acid response as well as preparation method and application of nano-drug
  • Nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen in acid response as well as preparation method and application of nano-drug
  • Nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen in acid response as well as preparation method and application of nano-drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] A preparation method of a nanomedicine capable of continuously releasing hydroxyl radicals and singlet oxygen in acid response, the synthesis process is as follows figure 1 shown, including the following steps:

[0074] (1) Preparation of hollow polydopamine materials

[0075] S1. Preparation of silica nanoparticles: 94.2 mL of ethanol, 16.2 mL of pure water, and 4.54 mL of ammonia water were mixed and stirred for 15 minutes, 5.4 mL of ethyl orthosilicate was added, and after stirring for 5 hours, the precipitate was collected by centrifugation at 8000 rpm for 10 minutes, and the precipitate was washed twice Then freeze-dried to obtain silica nanoparticles SiO 2 ;

[0076] S2. Dopamine coating on the surface of silica: resuspend 350 mg of silica nanoparticles in Tris-HCl buffer (pH 8.5, 10 mM), add 350 mg of dopamine hydrochloride, stir and react for 24 h and then centrifuge at 8000 rpm for 10 min to collect the precipitate , the precipitate was washed twice and then...

Embodiment 2

[0084] This example is basically the same as Example 1, except that, in the preparation of the acid-responsive nanocarrier material in step (2), the amount of hollow polydopamine used is 0.1 mg.

[0085] According to the above method, the nano-drug HPDA@CP@CPO that continuously releases hydroxyl radicals and singlet oxygen in acid response as described in Example 1 was prepared.

Embodiment 3

[0087] This example is basically the same as Example 1, except that, in the preparation of the acid-responsive nanocarrier material in step (2), the amount of hollow polydopamine used is 2 mg.

[0088] According to the above method, the nano-drug HPDA@CP@CPO that continuously releases hydroxyl radicals and singlet oxygen in acid response as described in Example 1 was prepared.

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Abstract

The invention discloses a nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen in an acid response manner as well as a preparation method and application of the nano-drug, and belongs to the field of nano-materials. The acid-responsive nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen is prepared by the following steps: (1) preparing a hollow polydopamine material; (2) preparing an acid response nano carrier material; and (3) preparing the nano-drug capable of continuously releasing hydroxyl free radicals and singlet oxygen in an acid response manner. According to the invention, the acid-responsive nano-carrier material is synthesized, and the biological enzyme is combined to the surface or the interior of the carrier material in a chemical bonding or physical adsorption manner, so that the acid-responsive nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen is obtained. The invention provides a nano-drug capable of responding to a tumor acid environment and continuously releasing free radicals, a cascade reaction can be carried out under an acidic condition to generate a large amount of hydrogen peroxide and further generate a large amount of < 1 > O < 2 > and. OH, and the nano-drug has an efficient anti-tumor effect.

Description

technical field [0001] The invention relates to the technology in the field of biomedical materials, and relates to the field of nanomaterials, in particular to a nanomedicine capable of continuously releasing hydroxyl radicals and singlet oxygen in response to an acid, and a preparation method and application thereof. Background technique [0002] At present, the prevention and control of malignant tumors is a key problem for the global health system to overcome. Among them, breast cancer is the number one "killer" threatening women's health and one of the malignant tumors with the highest incidence. 25% of new cases of tumor. Although the traditional breast cancer treatment method is a clinically mature treatment mode and can also inhibit breast cancer to a certain extent, due to its rapid development and poor prognosis, surgical therapy is not effective for patients who may have cancer cell metastasis. Although the curative effect has been improved after radiotherapy, du...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K33/34A61K47/34A61K47/32A61K47/59A61K47/58A61P35/00C08G81/02
CPCA61K38/44A61K33/34A61K47/34A61K47/32A61K47/59A61K47/58A61P35/00C12Y111/01009C08G81/024A61K2300/00Y02A50/30
Inventor 俞思明梁卓莹林褀薛巍
Owner JINAN UNIVERSITY
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