Method for measuring contents of glucose and xylose in sugar solution and tobacco extract

A technology of glucose and sugar solution, which is used in measurement devices, preparation of test samples, and color/spectral characteristics measurement, etc., can solve the problems of improving the accuracy of sugars, high danger of concentrated sulfuric acid, and poor color rendering effect of glucose. , to achieve excellent accuracy and accurate detection effect

Pending Publication Date: 2022-05-24
YUNNAN TOBACCO QUALITY SUPERVISION MONITORING STATION
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the existing phloroglucinol method has poor color development effect on glucose, the existing ultraviolet-visible chromatography method is difficult to realize the simultaneous determination of two or more sugars, concentrated sulfuric acid is more dangerous to the human body, and alkaloids and other substances in tobacco Interference measurement results The accuracy of the determination of sugars in tobacco by ultraviolet-visible chromatography needs to be improved. The first aspect of the present invention provides a method for measuring the content of glucose and xylose in the sugar solution. The second aspect of the present invention provides a A method for measuring the content of glucose and xylose in tobacco extract

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  • Method for measuring contents of glucose and xylose in sugar solution and tobacco extract
  • Method for measuring contents of glucose and xylose in sugar solution and tobacco extract
  • Method for measuring contents of glucose and xylose in sugar solution and tobacco extract

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Experimental program
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Effect test

Embodiment 1

[0073] Embodiment 1 measures the method for the content of glucose and xylose in self-made monosaccharide, mixed sugar solution

[0074] (1) Preparation of standard solution: Accurately prepare 10.00mmol / L glucose and xylose reserve mother liquor, and obtain different monosaccharide standard solutions with a concentration range of 0.01-2mmol / L after dilution; prepare glucose standard solution and xylose standard The equal volumes of the solutions are randomly mixed to obtain a mixed sugar solution of known concentration;

[0075] (2) Hydrolysis reaction: pipette 1 mL of monosaccharide standard solution or mixed sugar solution prepared in step (1) into a 25 mL stoppered colorimetric tube, add 4 mL of concentrated hydrochloric acid and 1 mL of glacial acetic acid, shake well and place in a boiling React in a constant temperature water bath for 40 minutes, take out the test tube immediately after the reaction is completed, put it in cold water to cool for 5 minutes, and carry out...

Embodiment 2

[0091] Embodiment 2 measures the content of glucose and xylose in tobacco products

[0092] (1) Preparation of tobacco extract: Weigh 0.1±0.0001g of tobacco sample into a 50mL Erlenmeyer flask with a stopper, add 20mL of pure water, cover the stopper, and oscillate at a constant temperature water bath oscillator at 30±0.5°C at a speed of 200rpm Extract for 30min. After the extraction, filter with rapid qualitative filter paper, discard the 2-3mL filtrate obtained by the initial filtration, and collect the subsequent liquid for later use to obtain the tobacco sample solution, namely the tobacco extract.

[0093] (2) Hydrolysis reaction: Pipette 1 mL of the prepared monosaccharide standard solution or mixed sugar solution or tobacco extract into a 25 mL stoppered colorimetric tube, add 4 mL of concentrated hydrochloric acid and 1 mL of glacial acetic acid, shake well and place in a boiling React in a constant temperature water bath for 40 minutes, take out the test tube immedia...

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Abstract

The invention discloses a method for determining the content of glucose and xylose in a sugar solution and a tobacco extract. The determination method is an ultraviolet-visible spectrum method. When the sugar solution is analyzed, (1) standard solution preparation and (2) hydrolysis reaction are carried out; (3) chromogenic reaction; (4) establishing a standard curve or a general model; (5) sample detection; and (6) outputting a result. The method for analyzing the tobacco extract comprises the following steps: (1) preparing the tobacco extract; (2) hydrolysis reaction; (3) chromogenic reaction; (4) establishing a standard curve or a general model; (5) sample detection; and (6) outputting a result. The phloroglucinol color developing agent is adopted, the color developing step is carried out after the hydrolysis step, and the hydrolysis adopts the mixed acid of concentrated hydrochloric acid and glacial acetic acid under the normal temperature condition, so that the phloroglucinol method is used for detecting the glucose for the first time, the glucose and the xylose can be simultaneously determined, and when the phloroglucinol method is used for detecting the glucose and the xylose in a sugar solution and a tobacco leaching solution, the detection accuracy is greatly improved. Operation is rapid and simple, detection efficiency is high, and accuracy is high.

Description

technical field [0001] The invention relates to the field of quality analysis of tobacco and tobacco products, in particular to a method for measuring the content of glucose and xylose in sugar solution and tobacco extract. Background technique [0002] Sugars in tobacco can be pyrolyzed under combustion conditions to produce various nascent compounds, thereby affecting the aroma and taste of cigarettes, and different sugar components will have different effects. Studies have shown that the content of glucose in cigarettes can enrich the aroma of cigarettes and improve the roundness of smoke; the content of fructose can help improve the extension and sweetness of tobacco leaves and reduce miscellaneous gas; the content of xylose can help improve the moistness and taste of smoke The sweet taste makes the aroma texture more delicate and smooth. Therefore, an accurate and rapid monosaccharide analysis and detection method is an important prerequisite for the quality control an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33G01N1/28G01N1/40
CPCG01N21/78G01N21/33G01N1/28G01N1/4044
Inventor 彭丽娟李宇赵璐何亮张克灿刘宇晨
Owner YUNNAN TOBACCO QUALITY SUPERVISION MONITORING STATION
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