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Plant methyl jasmonate in-situ detection method based on plate electrode biosensor

A technology of methyl jasmonate and biosensors, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of inability to accurately reflect the real-time changes of MeJA, plant damage, etc., and achieve the effects of good acquisition, low detection limit, and high sensitivity

Inactive Publication Date: 2022-05-20
NANTONG UNIVERSITY
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  • Abstract
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Problems solved by technology

[0003] At present, the quantitative detection methods of MeJA include enzyme-linked immunosorbent assay (ELISA), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), electrochemical methods, etc.; all of the above methods need to collect plant samples for separation and purification , then testing will not only cause damage to the plant, but also the results cannot accurately reflect the real-time changes in the content of MeJA in the plant
Electrochemical immunosensor is an electrochemical sensing technology based on antigen-antibody specific interaction, which combines the selectivity of antibodies and the sensitivity of electrochemical techniques, and has the advantages of portability, low cost, and easy miniaturization. In the prior art, there is no mature method or process for applying biosensors to the quantitative detection of MeJA in plants, and there are no relevant literature reports

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  • Plant methyl jasmonate in-situ detection method based on plate electrode biosensor
  • Plant methyl jasmonate in-situ detection method based on plate electrode biosensor
  • Plant methyl jasmonate in-situ detection method based on plate electrode biosensor

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Embodiment Construction

[0022] The technical solution of the present invention will be further described below according to multiple embodiments. In the description of this specification, the content of each embodiment means that the specific technical features described in conjunction with it are included in at least one embodiment of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the described specific technical features may be combined in any one or more implementations or examples in a proper manner.

[0023] 1. Preparation method of plate electrode biosensor

[0024] Based on a flat plate electrode, a three-electrode system is formed by wrapping a silver wire and a platinum wire on a portable clip (the clip can be reused many times), in which Ag / AgCl is the reference electrode, and the platinum wire is the counter electrode. The flat electrode modified by iron, graphene oxide, Nafi...

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Abstract

The invention provides a plant methyl jasmonate in-situ detection method based on a plate electrode biosensor, which realizes in-situ online detection of methyl jasmonate in a plant through the plate electrode biosensor. Compared with the traditional mass spectrum chromatography, the method disclosed by the invention does not need in vitro, has no essential harm to plants, has the characteristics of simplicity in operation, high sensitivity, high accuracy, convenience in carrying and the like, can realize dynamic acquisition of jasmonic acid methyl ester at different parts of plant leaves, and provides technical support for research of jasmonic acid methyl ester in plants.

Description

technical field [0001] The invention relates to a biological detection method, in particular to an in-situ detection method of plant methyl jasmonate based on a flat electrode biosensor. Background technique [0002] Methyl jasmonate (MeJA) is a plant endogenous hormone, which acts as a growth regulator, regulates the entire growth and development process of plants from seed germination to senescence, and also acts as a defense signaling molecule that activates plant stress responses. In agricultural production, exogenous application of MeJA can promote the synthesis of anthocyanins and phenolic compounds in fruits, which helps to improve the color and antioxidant capacity of fruits. In addition, MeJA treatment can also alleviate the adverse effects of agricultural products caused by mechanical damage during transportation or storage after harvest. However, due to the low content of MeJA in plants, usually between 0.01-1500 ng / g, there is an urgent need for a simple, sensit...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/3277G01N27/3278
Inventor 孙利军姚登兵张亚莉朱新宇孙玲张芝瑶
Owner NANTONG UNIVERSITY
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