Drug-loaded microspheres for targeted inhibition of TNBC cell activity, and preparation method and application thereof
A cell activity, drug-loaded microsphere technology, applied in the field of molecular biology, can solve problems such as low inhibition efficiency, and achieve the effect of easy operation, stable structure, and promotion of phagocytosis
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[0035] In the second aspect, the present application also provides a method for preparing drug-loaded microspheres targeted to inhibit the activity of TNBC cells, including the following steps, using CPADB, EDC and sulfo-NHS to react to obtain an intermediate product, using NH 2 -1000DA-COOH reacts with the intermediate product and adds hydrogen chloride to obtain COOH-PEG-CPADB, dissolve COOH-PEG-CPADB in anhydrous dioxane, add BMA and V501 and seal it with a diaphragm, then pass N 2 , react at 65-75°C for 16-20h to obtain COOH-PEG-CPADB-[co-BMA], add PDSMA and V70 to COOH-PEG-CPADB-[co-BMA] and seal it with a diaphragm, then pass N 2 , reacted at 65-75°C for 16-20h to obtain COOH-PEG-CPADB-[co-BMA-co-PDSMA], that is, to obtain PC-coBP microspheres; dissolve the PC-coBP microspheres and take them out after standing The clear liquid is mixed with the loading agent to obtain a mixture, and water is added to the mixture, followed by vortex and ultrasonic treatment to obtain a cr...
Embodiment 1
[0043] This example provides a drug-loaded microsphere for targeted inhibition of TNBC cell activity, including a CD24 aptamer and a carrier, the CD24 aptamer is located on the surface of the carrier, the carrier is a composite microsphere, and the composite microsphere includes a microsphere body and a loading agent. The microsphere body is PC-coBP microspheres, and the loading agent includes NF2 knockout plasmid, YAP overexpression plasmid, ferroptosis agonist Erastin and 1-2M2ge, NF2 knockout plasmid, YAP overexpression plasmid and PC-coBP microspheres The weight ratio is 1.5:1.5:4.
[0044] This embodiment also provides a preparation method of drug-loaded microspheres targeting the inhibition of TNBC cell activity, including the following steps, using CPADB, EDC and sulfo-NHS to react to obtain an intermediate product, using NH 2 - PEG-COOH reacts with the intermediate product and adds hydrogen chloride to obtain COOH-PEG-CPADB, dissolve COOH-PEG-CPADB in anhydrous dioxane...
Embodiment 2
[0046]This example provides a drug-loaded microsphere for targeted inhibition of TNBC cell activity, including a CD24 aptamer and a carrier, the CD24 aptamer is located on the surface of the carrier, the carrier is a composite microsphere, and the composite microsphere includes a microsphere body and a loading agent. The microsphere body is PC-coBP microspheres, and the loading agent includes NF2 knockout plasmid, YAP overexpression plasmid, ferroptosis agonist Erastin and 1-2M2ge, NF2 knockout plasmid, YAP overexpression plasmid and PC-coBP microspheres. The weight ratio is 2:2:5.
[0047] This embodiment also provides a preparation method of drug-loaded microspheres targeting the inhibition of TNBC cell activity, including the following steps, using CPADB, EDC and sulfo-NHS to react to obtain an intermediate product, using NH 2 - PEG-COOH reacts with the intermediate product and adds hydrogen chloride to obtain COOH-PEG-CPADB, dissolve COOH-PEG-CPADB in anhydrous dioxane, ad...
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