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Application of bile bacteria as diagnosis and prognosis marker of porta hepatic cholangiocarcinoma

A marker and cholangiocarcinoma technology, which is applied in the field of the use of bile bacteria as a diagnostic and prognostic marker for hilar cholangiocarcinoma, can solve problems such as unclear development and prognosis, achieve good clinical application prospects and improve the effect of quality of life

Active Publication Date: 2022-05-13
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bile microbiota, including bacteria and fungi, has not yet been studied and reported on the bile microbiota in patients with hilar cholangiocarcinoma. Whether the bile microbiota is related to the development and prognosis of hilar cholangiocarcinoma is unclear

Method used

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  • Application of bile bacteria as diagnosis and prognosis marker of porta hepatic cholangiocarcinoma
  • Application of bile bacteria as diagnosis and prognosis marker of porta hepatic cholangiocarcinoma
  • Application of bile bacteria as diagnosis and prognosis marker of porta hepatic cholangiocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Screening for the differential flora in the bile of patients with hilar cholangiocarcinoma

[0070] 1. Collection of samples

[0071] A total of 54 research subjects were collected in this study, of which 35 patients with hilar cholangiocarcinoma (hilar CCA) and 19 patients with benign biliary tract disease (benign BTD) were enrolled. The clinical information of the 54 patients is shown in Table 1. Show;

[0072] Inclusion criteria for patients with hilar cholangiocarcinoma: ①The cholangiocarcinoma of the common hepatic duct and the left and right hepatic ducts; ②According to two or more preoperative CT, MRI, and color Doppler ultrasound examinations, it is clear that there is a hepatic hilar space-occupying; Pathology confirmed hilar cholangiocarcinoma. ④ Relatively complete clinical and pathological data.

[0073] The exclusion criteria for patients with hilar cholangiocarcinoma were: ① metastatic cancer formed by duodenum, pancreas, and ampullary tumors ...

Embodiment 2

[0086] Example 2 Verification of the diagnostic efficiency of differential microorganisms in diagnosing hilar cholangiocarcinoma

[0087] Random forest analysis was performed based on LEfSe results, and the bacteria with good blocking ability were sorted according to the obtained average precision value (see figure 2 ), from which the abundance of 5 biliary bacteria f_Bacillaceae, g_Herbaspirillum, g_Anoxybacillus, g_Xylophilus, o_Verrucomicrobiales was screened, and according to the working abundance of f_Bacillaceae, g_Herbaspirillum, g_Anoxybacillus, g_Xylophilus, o_Verrucomicrobiales, the R package "pROC" was used to perform the C sink Analyze, draw the ROC curve, analyze the AUC value, sensitivity and specificity of the 5 kinds of bile bacteria f_Bacillaceae, g_Herbaspirillum, g_Anoxybacillus, g_Xylophilus, and / or o_Verrucomicrobiales obtained after the screening respectively as detection variables, to judge the effect of the bile bacteria on the liver Diagnostic perform...

Embodiment 3

[0091] Example 3 Screening of bile microbial markers related to the prognosis of hilar cholangiocarcinoma

[0092] R software (version 3.6.2) was used to conduct COX regression univariate analysis to screen the bile microorganisms associated with the survival of patients with hilar cholangiocarcinoma, and bile microorganisms with P<0.05 were considered to have an impact on the survival of patients with hilar cholangiocarcinoma According to the median risk score, patients with hilar cholangiocarcinoma were divided into high-risk group (high score) and low-risk group (low score), and then the KM (Kaplan-Meier) curve was drawn using GraphPad Prism 8.0, According to the obtained results, the differences in survival time between the high-risk group and the low-risk group were compared.

[0093]After screening, it was found that the abundance of Methylobacterium_komagatae was significantly negatively correlated with the survival of patients with hilar cholangiocarcinoma (P=0.0129670...

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Abstract

The invention discloses application of bile bacteria as a diagnosis and prognosis marker of porta hepatic cholangiocarcinoma, it is found for the first time that fBacillus, gHerbaspiril, gAnxyBacillus, gXylophilus and / or oVerrubmicrobiales can be used for diagnosis of early porta hepatic cholangiocarcinoma, sMethylobacteriumkomagatae can be used for prediction of prognosis of porta hepatic cholangiocarcinoma, and it is found through verification that the application of the bile bacteria as the diagnosis and prognosis marker of porta hepatic cholangiocarcinoma has the advantages that the application of the bile bacteria as the diagnosis and prognosis marker of porta hepatic cholangiocarcinoma has the advantages that the application of the bile bacteria as the diagnosis and prognosis marker of porta hepatic cholangiocarcinoma is promoted; the marker has relatively high diagnosis efficiency and accuracy on diagnosis and prognosis of porta hepatis bile duct cancer, and has a good clinical application prospect.

Description

technical field [0001] The present invention belongs to the field of biomedical technology. Specifically, the present invention relates to the use of bile bacteria as a diagnostic and prognostic marker for hilar cholangiocarcinoma. More specifically, the diagnostic markers described in the present invention include f_Bacillaceae, g_Herbaspirillum, g_Anoxybacillus, g_Xylophilus , and / or o_Verrucomicrobiales, the prognostic marker of the present invention is s_Methylobacterium_komagatae. Background technique [0002] Cholangiocarcinoma (CCA) is a malignant tumor originating from hepatic duct epithelial cells, and its incidence rate ranks second in hepatobiliary malignant tumors, second only to hepatocellular carcinoma. Cholangiocarcinoma can be divided into intrahepatic cholangiocarcinoma (ICCA), hilar cholangiocarcinoma (HCCA) and distal cholangiocarcinoma (DCCA) according to the location of its onset. Among them, hilar cholangiocarcinoma (DCCA) most common. Hilar cholangio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/689C12Q1/04
CPCC12Q1/6886C12Q1/689C12Q2600/118
Inventor 陆荫英贾晓东卢姗姗李爽雷光林李因茵
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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