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Establishment of detection method of colon tissue PINK1/Parkin of rat dysbacteriosis diarrhea

A technology for dysbacteriosis and detection methods, used in biochemical equipment and methods, determination/inspection of microorganisms, measurement devices, etc.

Pending Publication Date: 2022-05-13
贵州中医药大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological protection of the PINK1 / Parkin pathway in many diseases has been widely confirmed, but there are few reports on the regulation of dysbiosis diarrhea. To observe the effect of Gegen Qinlian Decoction (GQT) on the expression level of PINK1 / Parkin in the colon tissue of rat model animals with dysbiosis diarrhea, and to explore the mechanism of GQT in treating diarrhea

Method used

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  • Establishment of detection method of colon tissue PINK1/Parkin of rat dysbacteriosis diarrhea
  • Establishment of detection method of colon tissue PINK1/Parkin of rat dysbacteriosis diarrhea
  • Establishment of detection method of colon tissue PINK1/Parkin of rat dysbacteriosis diarrhea

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] SD rats were taken daily according to the dosage and administered with cephradine 178.6mg.kg-1 and gentamicin sulfate 31.25mg.kg-1 mixed antibiotics to establish a model, once a day, for 7 consecutive days, at 9:00 a.m. every day -10:00 to complete the modeling. Evaluation was carried out after 7 days of continuous modeling. After the success of the model, Gegen Qinlian decoction was administered orally, and the crude drug amount was 3.51 g·kg-1).

[0053] After 14 days of treatment for rats, the rats were fasted for 6 hours, injected with anesthesia, randomly cut out the rat colon tissue with tissue scissors, removed excess fat around the tissue, rinsed it repeatedly with pre-cooled normal saline, and put filter paper Blot up the liquid, fix a part of the colon tissue in 4% paraformaldehyde solution for HE staining, and put the other part into a dry sterile centrifuge tube for subsequent experiments such as real-time fluorescent quantitative PCR method.

[0054] The c...

Embodiment 2

[0058] SD rats were taken daily according to the dosage and administered with cephradine 178.6mg.kg-1 and gentamicin sulfate 31.25mg.kg-1 mixed antibiotics to establish a model, once a day, for 7 consecutive days, at 9:00 a.m. every day -10:00 to complete the modeling. The evaluation was carried out after 7 days of continuous modeling, and Gegen Qinlian decoction was administered orally after the model was successful. (Based on the crude drug amount, it is 7.02g·kg-1).

[0059] After 7 days of treatment for rats, the rats were fasted for 6 hours, injected with anesthesia, randomly cut out the rat colon tissue with tissue scissors, removed excess fat around the tissue, rinsed it repeatedly with pre-cooled normal saline, and put filter paper Blot up the liquid, fix a part of the colon tissue in 4% paraformaldehyde solution for HE staining, and put the other part into a dry sterile centrifuge tube for subsequent experiments such as real-time fluorescent quantitative PCR method. ...

Embodiment 3

[0064] SD rats were taken daily according to the dosage and administered with cephradine 178.6mg.kg-1 and gentamicin sulfate 31.25mg.kg-1 mixed antibiotics to establish a model, once a day, for 7 consecutive days, at 9:00 a.m. every day -10:00 to complete the modeling. The evaluation was carried out after 7 days of continuous modeling, and Gegen Qinlian decoction was administered orally after the model was successful. (Calculation based on crude drug amount is 1.755g·kg-1 respectively).

[0065] After 21 days of drug treatment in rats, the rats were fasted for 6 hours, injected with anesthesia, randomly cut out the rat colon tissue with tissue scissors, removed excess fat around the tissue, rinsed it repeatedly with pre-cooled normal saline, and put filter paper Blot up the liquid, fix a part of the colon tissue in 4% paraformaldehyde solution for HE staining, and put the other part into a dry sterile centrifuge tube for subsequent experiments such as real-time fluorescent qu...

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Abstract

The invention discloses establishment of a detection method of colon tissue PINK1 / Parkin of rat dysflora diarrhea, and the detection method comprises the following steps: preparing a rat model with dysflora diarrhea, treating the rat model with a Gegen Qinlian decoction, performing HE dyeing and RT-PAR detection, and detecting the PINK1 / Parkin in the rat model with PINK1 / Parkin. It is determined that the radix puerariae qinlian decoction can reduce the expression level of mitochondrial autophagy PINK1 / Parkin in colon tissue of a rat suffering from the dysflora diarrhea, and the radix puerariae qinlian decoction can activate mitochondrial autophagy and remove damaged mitochondria in the tissue by influencing expression of PINK1 and Parkin, so that the effect of treating the dysflora diarrhea is achieved.

Description

technical field [0001] The invention relates to the establishment of a method for detecting PINK1 / Parkin in the colon tissue of rats with dysbacteriosis diarrhea, especially the detection of PINK1 / Parkin expression in the colon tissue of rat models of dysbacteriosis diarrhea by Gegen Qinlian Decoction by HE staining and RT-PAR Impact. Background technique [0002] Antibiotic-associated dysbacteriosis (Dysbacteriosis) refers to the intestinal flora disorder that occurs after the use of antibiotics. Its main clinical manifestation is diarrhea, which can also be accompanied by abdominal distension, abdominal pain, fever, nausea, and vomiting. If not treated in time, it can cause water-electrolyte acid-base imbalance and superinfection, and even lead to systemic inflammatory response syndrome, multi-organ inflammation, etc. functional failure etc. While Western medicine uses antibiotics to correct the primary disease, the main methods are microecological adjustment preparation...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851G01N21/01
CPCC12Q1/6851G01N21/01C12Q2521/107C12Q2531/113C12Q2545/107
Inventor 何光志张庚鑫杨光勇杜海洋苏钢田维毅王文佳涂小华聂捡刘丹陈墨陈未来
Owner 贵州中医药大学
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