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Plant nematode control method

A technology for plant nematodes and nematodes, applied in the fields of botanical equipment and methods, nematicides, plant protection, etc., can solve problems such as the dealkylation of nematodes that are not yet known, and achieve strong target specificity and high species broad spectrum. sexual effect

Active Publication Date: 2022-05-13
HAINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, it is not known whether DHCR24-mediated dealkylation occurs in C. elegans, or what function they might have had in this ancient organism

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Comparison of Nematode Steroid Metabolic Profiles Before and After DHCR24 Blocking

[0056] The present invention obtains Caenorhabditis elegans CeDHCR24 knockout mutant tm2141, the mutant is F52H2.6 / DHCR24 allele, the deletion range is 29539 to 30298, and the length is 760bp. This mutant allele lost exon 4 and part of exon 5. The tm2141 mutant can grow normally on the medium (NGM) containing 5mg / L cholesterol. Mutated anabolic cholesterol (1; figure 1 and figure 2 ) is metabolized to 7-dehydrocholesterol (2; figure 2 ), 7-enecholestanol (3; Lathosterol, figure 2 ), 4α-methylcholst-8(14)enol(4; figure 2 ), lophanol (5; figure 2 ) and lophenol (6; figure 2 ). Its sterol profile is very similar to that of wild Caenorhabditis elegans fed cholesterol (Chitwood, D.J., Lusby, W.R., Lozano, R., Thompson, M.J. & Svoboda, J.A. Sterol metabolism in the nematode Caenorhabditis elegans. Lipids, 1984, 19, 500-506) Similarly, it was suggested that DHCR24 is i...

Embodiment 2

[0059] Example 2 Causes its developmental arrest by blocking the endogenous phytosterol conversion pathway of nematodes

[0060] When cultured with sufficient cholesterol, tm2141 was able to complete the entire life cycle indistinguishable from wild-type C. elegans (N2) animals. After synchronization on cholesterol plates, L1 worms (see Caenorhabditis elegans life cycle: Fielenbach, N. & Antebi, A.C. elegans dauer formation and the molecular basis of plasticity. Genes & Development, 2008, 22, 2149-2165) were transferred to worms containing only gluten. Alcohol plate. After 48h at 25°C, 94% of wild-type worms grew to adults ( Figure 5 1 in the middle), while the development of tm2141 was significantly retarded, and only 20% of the worms reached the adult stage ( Figure 5 in 2). Progeny of tm2141 fed with sitosterol produced synchronized L1 worms. The worms were depleted of cholesterol and transferred to fresh sitosterol plates. After 48 hours of incubation at 25°C, 100% ...

Embodiment 3

[0062] Example 3 Causes growth arrest of nematodes after eating corresponding phytosterols by regulating sterol profiles in plants

[0063] Isotope labeling experiments showed that insect C24 and C25 hydrogens are essential for sterol dealkylation (Fujimoto, Y., Kimura, M., Takasu, A., Khalifa, F.A.M. & Ikekawa, N. Mechanism of stigmasteroldealkylation in insect. Tetrahedron Letters , 1984, 25, 1501–1504; Ikekawa, N., Morisaki, M. & Fujimoto, Y. Sterol metabolism in insects: dealkylation of phytosterol to cholesterol. Accounts of Chemical Research, 1993, 26, 139-146). Based on this, 24-ethylcholesterol 5,25(27)-dienol (clerosterol, CLE; 11, figure 1). CLE occurs naturally in plants but lacks the C25 hydrogen (for structure and mass spectra see figure 1 ).

[0064] Exogenous sterols and their nuclear-modified intermediates are major constituents of worms ( image 3 ). Although no cholesterol was detected in animals, CLE was taken up and converted to 24-ethylcholesta-5,7,25...

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Abstract

The invention provides a method for preventing and treating plant nematodes. According to the method, plant nematodes are fed with phytosterol, and the phytosterol does not contain cholesterol and sitosterol. The DHCR24-dependent nematode prevention and control method provided by the invention is completely different from known genetic engineering strategies of crop rotation, chemical nematicides or specific protease of nematodes, and has stronger target specificity and higher species broad spectrum. According to the invention, nematode control is realized by interfering steroid metabolism of nematodes or regulating steroid metabolism of plants, and specific control strategies can be developed for different nematode species or different crop strains. And a theoretical support and a technical platform are provided for realizing parasitic nematode prevention and control by regulating sterol metabolism in other species (such as livestock).

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preventing and controlling plant nematodes. Background technique [0002] Plant nematode disease is an important disease that seriously restricts the yield of crops. Its damage exceeds that of bacteria and viruses, and is second only to fungal diseases. It causes about US$125 billion in world agricultural production losses every year (Chitwood, Biochemistry and function of nematode steroids, Critical Reviews in Biochemistry and Molecular Biology, 1999, 34(4) 273-284). Common nematode control methods include crop rotation and chemical nematicides. Crop rotation is mainly through the rotation of susceptible crops and lightly susceptible crops, which can reduce the insect population density to a certain extent. However, some cyst nematodes survive in the soil for more than 10 years; and some nematodes (such as root-knot nematode) have a wide host range, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00
CPCA01G13/00A01P5/00A01N65/08
Inventor 路延笃周文序
Owner HAINAN UNIVERSITY
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