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MRFFT2 cell construction method

A construction method and cell technology, applied in the biological field, can solve problems such as T cell self-protection technology, and achieve the effects of strong target specificity, increased lethality, and high safety

Inactive Publication Date: 2019-01-04
BEIJING DCTY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] None of the above schemes considers the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful tumor immune microenvironment

Method used

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  • MRFFT2 cell construction method
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Embodiment Construction

[0058]The present invention is described below through specific embodiments. Unless otherwise specified, the technical means used in the present invention are methods known to those skilled in the art. In addition, the embodiments should be considered as illustrative rather than limiting the scope of the invention, the spirit and scope of which is defined only by the claims. For those skilled in the art, on the premise of not departing from the spirit and scope of the present invention, various changes or modifications to the material components and dosage in these embodiments also belong to the protection scope of the present invention.

[0059] 1. Epitope prediction

[0060] 1) Use the peripheral blood of lung cancer patients for ctDNA sequencing and HLA typing detection;

[0061] 2) Use software to analyze the sequencing information: compare the ctDNA sequencing results with the genome of normal cells, and screen out the mutation sites;

[0062] 3) Take the mutated amino...

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Abstract

The invention relates to an MRFFT2 cell construction method, a mutation polypeptide is screened through the predication of an epitope, the sequence of an expression gene of the mutation polypeptide isconnected and synthesized; meanwhile, an MVA virus vector is constructed, an MVA virus is packaged, an APC (Antigen Presenting Cell) is transfected, the transformation of a specific MV cell is finished, the APC is cultured in vitro together with a PBMC (Peripheral Blood Mononuclear Cell) separated from the peripheral blood, the effective polypeptide is screened, a common T cell is transformed into an RFF cell having more accurate lethality through the second impact stimulated by the accurate effective polypeptide; the transformation is conducted through the TCR-T technical principle; an immunosuppressive signal of the transformed T cell is closed by using an antibody drug in vitro, the specific lethal T cell is protected accurately against in-vivo suppression, and the lethality of the T cell on a tumor cell is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for constructing MRFFT2 cells. Background technique [0002] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proved to be ineffective, while NK, CAR-NK, TIL and other cell technologies have yet to mature. CAR- T cells also have shortcomings in safety and solid tumor therapy. In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0003] The above treatment methods are immature, especially the in vitro induction of DC cells and the te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/90C12N5/10
CPCC07K14/7051C12N5/0638C12N15/907C12N2510/00C12N2800/80C12N2810/10
Inventor 焦顺昌张嵘宋玉洁周子珊解佳森袁翰李营营
Owner BEIJING DCTY BIOTECH CO LTD
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