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Reagent composition for b-all/lbl immunotyping and application thereof

A technology of immunotyping and composition, which is applied in the field of reagent composition for immunophenotyping of B-lymphoblastic leukemia/lymphoma, can solve the problems of poor practicality, achieve good positive predictive value and negative predictive value, and high sensitivity and specificity, and the effect of improving analytical ability

Active Publication Date: 2022-07-05
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Less practical

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  • Reagent composition for b-all/lbl immunotyping and application thereof
  • Reagent composition for b-all/lbl immunotyping and application thereof
  • Reagent composition for b-all/lbl immunotyping and application thereof

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Embodiment 1

[0058] Example 1 Preparation of reagents

[0059] The present invention provides antibody combinations, a total of 20 kinds of antibodies, the antibodies and compatible fluorescein are configured according to the combinations in Table 3, including: anti-CD22-SB436, anti-CD20-eFluor450, anti-CD21-BV480, anti-CD45-BV510, anti-CD10 -BV605, anti-CD19-BV650, anti-CD24-BV711, anti-CD123-BV785, anti-CD65-FITC, anti-CD15-cFluorB548, anti-CD13-PE, anti-lambda-PE-Dazzle594, anti-HLA-DR-PE-Cy5, anti- CD34-PerCP-Cy5.5, anti-CD58-PerCP-Vio700, anti-CD81-PE-Cy7, anti-CD66a / c / e-AF647, anti-Kappa-eFluorR720, anti-CD38-APC-Fire750, anti-CD25-APC-Fire810.

[0060] Configure antibody combination: Take the above 20 kinds of antibodies, mix and pack them in a container according to the dosage determined in the pre-experiment, and use them for immunophenotyping and labeling of B-ALL / LBL samples.

[0061]The above-mentioned antibodies can be directly purchased commercially, and the antibodies of th...

Embodiment 2

[0063] Example 2 Immunophenotype analysis of B-ALL / LBL by 20-color antibody combination flow cytometry

[0064] 1. Main experimental materials and instruments

[0065] 1. Materials: 10×PBS buffer, hemolysin for flow cytometer (BD company);

[0066] 2. Instrument: CytekNL-3000 full spectrum flow cytometer, equipped with 405nm, 488nm, 635nm three lasers, 38 fluorescence detectors. Tabletop low-speed centrifuge, vortex mixer.

[0067] 2. Method

[0068] 1. Sample collection:

[0069] Immediately place 1-3 mL of the obtained human bone marrow fluid in a heparin anticoagulant tube and invert several times quickly to prevent the specimen from coagulating. Various cells such as pleural and ascites fluid and lavage fluid should be sent to the laboratory as soon as possible after collection, and the specimens should be stored at 4℃. The flow cytometry (FCM) detection must be completed within 48 hours, and the operation should be performed according to the instructions.

[0070] 2...

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PUM

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Abstract

The invention provides a reagent composition for B-lymphoblastic leukemia / lymphoma (B-ALL / LBL) immunophenotyping and MRD monitoring, the reagent composition comprising a group of 20 antibody combinations. The invention optimizes the antibody combination and the corresponding antibody fluorescent labeling combination and the result interpretation method, and only needs to use 20 kinds of antibodies to load a tube of cells at one time, which can comprehensively and efficiently perform subtype typing of B lymphoblastic leukemia / lymphoma , and can predict some recurrent genetic abnormalities B‑ALL / LBL, and has high sensitivity and specificity for the diagnosis of BCR / ABL1 genotype. At the same time, the leukemia-related immunophenotype (LAIP) that can be used for post-treatment minimal residual disease (MRD) monitoring in this combination can be determined, and MRD monitoring and detection after CAR-T therapy can be performed.

Description

technical field [0001] The invention relates to the field of antibody medicine, in particular to a group of reagent compositions for B lymphoblastic leukemia / lymphoma immunotyping and application thereof. Background technique [0002] Lymphoblastic leukemia / lymphoma (ALL / LBL) is a malignant tumor originating from precursor lymphocytes that can involve the bone marrow and peripheral blood (ALL), as well as lymph nodes, thymus, or extranodal tissue ( LBL). Usually, the diagnosis of LBL is more appropriate when the lesion is mainly in the form of a mass and does not involve or rarely involves peripheral blood or bone marrow; when a large number of blast cells are detected in PB or BM as the main manifestation, the diagnosis of ALL is more appropriate. Using immunological methods, ALL / LBL can be divided into two categories: B cells and T cells. The clinical manifestations, treatment plans and prognosis of the two types of diseases are different, and the accurate diagnosis and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/574G01N33/58G01N15/14
CPCG01N33/6893G01N33/6872G01N33/577G01N33/57426G01N33/57407G01N33/57484G01N33/582G01N15/14G01N2015/1486G01N33/56972G01N2333/70503G01N2015/1006G01N2015/1402G01N15/1459G01N2015/1488G01N2800/7028
Inventor 刘艳荣王亚哲
Owner PEOPLES HOSPITAL PEKING UNIV
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